The UltiMate® 3000 x2 Dual Titanium system is a fully biocompatible analytical LC system for advanced multidimensional (MD) protein analysis and purification. It supports advanced biochromatography schemes such as application switching and automated MD chromatography, for example, purification and analysis of biotherapeutics like monoclonal antibodies in a single method.
- Titanium pumps and PEEK™ flow path for full biocompatibility
- SmartFlow™ technology for precise and accurate flow rates from 0.2 to 10 mL/min
- Low-pressure proportioning at superior accuracy and precision
- Dual ternary pumps for true parallel, tandem or multidimensional chromatography
- Convenient solvent selection for eluent preparation and system flushing
- Ultralow carryover, high precision auto-injections
- Chromeleon® CMS—powerful, easy-to-use and provides tools for 21 CFR Part 11 compliance
Titanium pumps and PEEK flow paths make the UltiMate 3000 Titanium system fully biocompatible, ensuring biological integrity of proteins during separation. It features dual gradient pumps in a single module to support parallel, tandem, and multidimensional separations for highest flexibility and productivity while saving money and space.
| Biocompatible x2 Dual Analytical |
|---|
| Maximum system pressure | 35 MPa (5,750 psi) |
| PUMP |
|---|
| Recommended pump | DGP-3600AB |
| Flow rate range (recommended) | 0–10 mL/min (0.1–10 mL/min) |
| Flow accuracy | ±0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD at 1 mL/min, whichever is greater |
| Pressure range | 2–50 MPa (290–7250 psi) |
| No. of eluent lines | 6 (2 x 3) |
| Gradient delay volume | 690 µL (360 µL with micro flow kit) |
| Solvent degassing | External (optional) |
| AUTOSAMPLER |
|---|
| Recommended | WPS-3000TBPL Analytical |
| Injection volume range (recommended) | 0.1–250 µL
(settable: 0.001–250 µL) |
| Injection volume accuracy | N/A |
| Injection volume precision | <0.25% RSD at 5 µL in full loop <0.3% RSD at 5 µL (with 20 µL partial loop each) |
| Linearity | Corr. coeff. >0.9999 RSD <0.5% at 5–30 µL, caffeine in water |
| Carry over | <0.02% for caffeine with external wash |
| Injection cycle time | <60 s for 5 µL full-loop injection, <90 s for 5 µL partial-loop injection |
| Optional thermostating | 4–45 °C, at least 22 °C below ambient |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000SD |
| No. of switching valves | Optional, up to 2 PEEKTM valves |
| Recommended eluent pre-heater volume | 7 µL (0.01" i.d.) biocompatible |
| Temperature range | 5 °C to 80 °C (max. 18 °C below ambient) |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| Heat-up/cool-down time | Typicallly: 15 min from 20 °C to 50 °C/ 15 min from 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended detector | VWD-3100 |
| Detector type | Variable Wavelength |
| Number of channels | 1 |
| Wavelength range | 190–900 nm |
| Max. data collection rate | 100 Hz |
| Recommended flow cell | Analytical, PEEK, 11 µL, 10 mm path |
| Noise | <±3.5 µAU at 254 nm |
| Drift | <0.1 mAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following LC modules and accessories represent a typical UltiMate 3000 x2 Dual Titanium system configuration.
| Hardware |
|---|
| SRD-3600 UltiMate 3000 Integrated Solvent and Degasser Rack, 6 Channels |
5035.9230 |
| DGP-3600AB UltiMate 3000 Biocompatible Dual Ternary Low-Pressure Proportioning Analytical Pump System |
5037.0014 |
| WPS-3000TBPL Analytical - UltiMate 3000 Biocompatible Thermostatted Analytical Pulled-Loop Well Plate Autosampler |
5823.0020 |
| TCC-3000SD Thermostatted Column Compartment |
5730.0010 |
| VWD-3400RS - UltiMate 3000 Rapid Separation Four Channel Variable Wavelength Detector (Without Flow Cell) |
5074.0010 |
| Accessories |
|---|
| Valve Actuator Kit HP for Right Side of TCC-3000RS/SD, Pressure < 413 bar (6000 psi)
|
6730.0003 |
| Valve Actuator Kit HP for Left Side of TCC-3000RS/SD, Pressure < 413 bar (6000 psi)
|
6730.0004 |
| Pod for 2-Position 10-Port HP Valve, Biocompatible, Pressure < 345 bar (5000 psi) |
6723.9023 |
| Analytical Flow Cell for VWD-3000 Series, PEEK, 11 µL Volume, 10 mm Pathlength |
6074.0200 |
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