The UltiMate® 3000 x2 Dual Analytical system is the ideal choice to maximize sample throughput for your conventional HPLC and UHPLC separations. The system combines the flexibility of ternary solvent selection with the productivity and performance of x2 Dual LC at system pressures up to 620 bar. The modular design is easily customized to your applications and sample preparation needs.
- SmartFlow® technology for precise and accurate flow rates from 0.2 to 10 mL/min
- UHPLC compatibility with operating pressures up to 62 MPa (9000 psi)
- Optimized extracolumn volume for superior separation efficiency
- Ultralow carryover, high precision μL auto-injections
- Wide temperature range, thermostatted control of samples and columns
- UltiMate 3000 x2 Dual Titanium system available
Intelligent x2 Dual LC solutions save time and effort. Parallel and tandem configurations increase sample throughput by up to 100%, by automating two applications in one run. Multidimensional separations provide resolution impossible with single-column methods. On-line sample preparation saves time and reduces complexity, sample loss, and matrix interferences.
| PUMP |
|---|
| Recommended pump | DGP-3600SD |
Flow range (settable
range) | 0.2–10 mL/min (0.001–10 mL/min) |
| Flow accuracy | ±0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure range | 2–62 MPa (290–9,000 psi) |
| No. of eluent lines | 6 (2 x 3) |
| Gradient delay volume | 690 µL |
| Solvent degassing | External (optional) |
| AUTOSAMPLER |
|---|
| Recommended | WPS-3000SL Analytical |
| Injection volume range (recommended) | 0.01 – 100 µL (1–100 µL) Optional: 0.2 – 25 µL, 1.5 – 250 µL, 1.5 – 500 µL |
| Injection volume accuracy | ±0.5% at 50 and 90 µL |
| Injection volume precision | <0.25% RSD at 5 µL (typically <0.15% RSD), caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine in water |
| Carry over | <0.004% for caffeine with external wash at 75 bar |
| Injection cycle time | <15 s for 5 µL |
| Optional thermostating | 4–45 °C, at least 22 °C below ambient |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000SD |
| No. of switching valves | Optional, up to 2 |
| Recommended eluent pre-heater volume | 7 µL (0.01" i.d.) |
| Temperature range | 5 °C to 80 °C (max. 18 °C below ambient) |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| Heat-up/cool-down time | Typicallly: 15 min from 20 °C to 50 °C/15 min from 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended detector | DAD-3000 |
| Detector type | Diode Array |
| Number of channels | 8 plus 3D field |
| Wavelength range | 190–800 nm |
| Max. data collection rate | 100 Hz |
| Recommended flow cell | Analytical, 13 µL, 10 mm path |
| Noise | <±8 µAU at 254 nm (wide slit) |
| Drift | <1 mAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following LC modules represent a typical x2 Dual Analytical system configuration for tandem analyses.
| Hardware |
|---|
| SRD-3600 UltiMate 3000 Integrated Solvent and Degasser Rack, 6 Channels |
5035.9230 |
| DGP-3600SD - UltiMate 3000 Dual-Gradient Analytical Pump |
5040.0061 |
| WPS-3000TRS Rapid Separation Wellplate Sampler, Thermostatted |
5840.0020 |
| TCC-3000SD Thermostatted Column Compartment |
5730.0010 |
| DAD-3000 - UltiMate 3000 Diode Array Detector (Without Flow Cell) |
5082.0010 |
| Accessories |
|---|
| Pod for 2-Position 10-Port HP Valve, SST Variant, Pressure < 413 bar (6,000 psi) |
6722.9023 |
| Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 13 µL Volume, 10mm Path Length |
6082.0100 |
| Viper UHPLC Fingertight Fitting and Capillary Kit for Online SPE on UltiMate 3000 x2 Dual Standard Systems |
6040.2802 |
| Viper UHPLC Fingertight Fitting and Capillary Kit for Tandem Operation on UltiMate 3000 x2 Dual Standard Systems |
6040.2804 |
| Viper UHPLC Fingertight Fitting and Capillary Kit for Application Switching on UltiMate 3000 x2 Dual Standard Systems |
6040.2806 |
| Viper UHPLC Fingertight Fitting and Capillary Kit for Automated Method Scouting on UltiMate 3000 Quaternary Standard Systems |
6040.2808 |
| Viper UHPLC Fingertight Fitting and Capillary Kit for Parallel Setup on UltiMate 3000 x2 Dual Standard Systems |
6040.2810 |
| Pod for 2-Position 6-Port HP Valve, SST Variant, Pressure < 413 bar (6000 psi) |
6722.9013 |
| Pod for 6-Position 7-Port HP Valve, Titanium Variant, 10-32 Fittings, Pressure < 413 bar/6000 psi
|
6722.9035 |
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