The UltiMate® 3000 Quaternary Micro system is the ideal choice for laboratories that need flexibility for microbore LC and LC/MS analyses. The selection of up to four solvents and quaternary gradient profiles accelerates method development and offers flexibility during automated system startup and shutdown.
- SmartFlow™ technology for precise and accurate flow rates from 10 to 2500 μL/min
- Quaternary low-pressure proportioning at superior accuracy and precision
- Convenient solvent selection for eluent preparation and system flushing
- Selection of choices and combinations of gradient profiles (linear, step, concave, convex)
- Ultralow carryover, high precision μL auto-injections
- Wide temperature range, thermostatted control of samples and columns
- Wide selection of high-performance detectors
The UltiMate 3000 Quaternary Micro system combines lowest detection limits, reduced baseline noise, and maximum flexibility. It is recommended as a micro LC front-end for LC/MS platforms and other micro LC applications. With its reduced system delay volume and high detection data rates, it is also suitable for fast and very fast microbore LC applications.
| Quaternary Micro |
|---|
| Maximum system pressure | 41 MPa (6,000 psi) |
| PUMP |
|---|
| Recommended pump | LPG-3400BM |
| Settable flow range (recommended) | 0–2500 µL/min
(10–2500 µL/min) |
| Flow accuracy | ±0.5% at 200 µL/min |
| Flow precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure range | 0.1–50 MPa (15–7,250 psi) |
| No of eluent lines | 4 |
| Gradient delay volume | 220 µL |
| Solvent degassing | Built-in, 4 channels |
| AUTOSAMPLER |
|---|
| Recommended sampler | WPS-3000SL Analytical |
| Injection volume range (recommended) | 0.01 – 100 µL (1–100 µL) Optional: 0.2 – 25 µL, 1.5 – 250 µL, 1.5 – 500 µL |
| Injection volume accuracy | ±0.5% at 50 and 90 µL |
| Injection volume precision | <0.25% RSD at 5 µL (typically <0.15% RSD), caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine in water |
| Carry over | <0.004% for caffeine with external wash at 75 bar |
| Injection cycle time | <15 s for 5 µL |
| Sample thermostating | 4–45 °C, at 22 °C below ambient (thermostatted sampler version) |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000SD |
| No. of switching valves | Optional, up to 2 |
| Recommended eluent pre-heater volume | 2 µL (0.005" i.d.) |
| Temperature range | 5 °C to 80 °C |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| DETECTOR |
|---|
| Recommended detector | VWD-3100 |
| Detector type | Variable Wavelength |
| Number of channels | 1 |
| Wavelength range | 190–900 nm |
| Max. data collection rate | 100 Hz |
| Recommended flow cell | Semi-micro flow cell, 2.5 µL, SST |
| Noise | < ±3.5 µAU at 254 nm |
| Drift | < 0.1 mAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following modules and accessories represent a typical UltiMate 3000 Quaternary Micro system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| SR-3000 UltiMate 3000 Solvent Rack without Degasser |
5035.9200 |
| LPG-3400M UltiMate 3000 Quaternary Low Pressure Proportioning Micro Pump |
5035.0045 |
| WPS-3000TRS Rapid Separation Wellplate Sampler, Thermostatted |
5840.0020 |
| TCC-3000SD Thermostatted Column Compartment |
5730.0010 |
| VWD-3400RS - UltiMate 3000 Rapid Separation Four Channel Variable Wavelength Detector (Without Flow Cell) |
5074.0010 |
| Accessories |
|---|
| Semi-micro Flow Cell for VWD-3000 Series, SST, 2.5 µL Volume, 7 mm Pathlength |
6074.0360 |
| Sample loop, 25 μL, for UltiMate 3000 WPS-3000SL Analytical and WPS-3000RS Autosampler Series, until S/N 8049346
|
6820.2415 |
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