The UltiMate® 3000 Quaternary Analytical system offers technical and practical benefits to almost any laboratory and application area. It supports the widest range of solvent options and gradient profiles, making method development easy. It is also compatible with UHPLC applications up to 62 MPa (9000 psi).
- SmartFlow® technology for precise and accurate flow rates from 0.2 to 10 mL/min
- Quaternary low-pressure proportioning at superior accuracy and precision
- Convenient solvent selection for eluent preparation and system flushing
- Multiple choices and combinations of gradient profiles (linear, step, concave, convex)
- Ultralow carryover, high precision auto-injections
- Wide temperature range, thermostatting of samples and columns
- Diode Array Detector for convenient method development at high detection sensitivity
- Biocompatible version available (UltiMate 3000 Titanium system)
The UltiMate 3000 Quaternary Analytical system provides remarkable retention time precision, accurate and precise eluent selection and proportioning efficiency, and excellent mixing performance for quaternary HPLC and UHPLC applications.
| PUMP |
|---|
| Recommended Pump | LPG-3400SD |
Flow Range (Settable
Range) | 0.2–10 mL/min (0.001–10 mL/min) |
| Flow Accuracy | ±0.1% |
| Flow Precision | <0.05% RSD or <0.01 min SD at 1 mL/min, whichever is greater |
| Pressure Range | 2–62 MPa (290–9,000 psi) |
| No. of Eluent Lines | 4 |
| Gradient delay volume | 690 µL |
| Solvent degassing | Built-in, 4 channels |
| AUTOSAMPLER |
|---|
| Recommended Autosampler | WPS-3000(T)SL Analytical |
| Injection Volume Range (recommended) | 0.01–100 µL (1–100 µL) Optional: 0.2–25 µL, 1.5–250 µL, 1.5–500 µL |
| Injection Volume Accuracy | ±0.5% at 50 and 90 µL |
| Injection Volume Precision | <0.25% RSD at 5 µL (typically <0.15% RSD), caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine in water |
| Carryover | <0.004% for caffeine with external wash at 75 bar |
| Injection Cycle Time | <15 s for 5 µL |
| Sample Thermostatting | 4–45 °C, at least 22 °C below ambient (thermostatted sampler version) |
| COLUMN COMPARTMENT |
|---|
| Recommended Column Compartment | TCC-3000SD |
| No. of Switching Valves | Optional, up to 2 |
| Recommended Eluent Preheater Volume | 7 µL (0.01"i.d.) |
| Temperature Range | 5 °C to 80 °C (max. 18 °C below ambient) |
| Temperature Accuracy | ±0.5 °C |
| Temperature Stability/Precision | ±0.1 °C |
| Heat-Up/Cool-Down Time | Typicallly: 15 min from 20 °C to 50 °C/ 15 min from 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended Detector | DAD-3000 |
| Detector Type | Diode Array |
| Number of Channels | 8 plus 3D field |
| Wavelength Range | 190–800 nm |
| Max. Data Collection Rate | 100 Hz |
| Recommended Flow Cell | Analytical, 13 µL, 10 mm path |
| Noise | <±8 µAU at 254 nm (wide slit) |
| Drift | <1 mAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following modules and accessories represent a typical UltiMate 3000 Quaternary Analytical system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| SR-3000 UltiMate 3000 Solvent Rack without Degasser |
5035.9200 |
| LPG-3400SD - UltiMate 3000 Quaternary Analytical Pump |
5040.0031 |
| WPS-3000TSL UltiMate 3000 Thermostatted Analytical Sampler |
5822.0020 |
| TCC-3000SD Thermostatted Column Compartment |
5730.0010 |
| DAD-3000 - UltiMate 3000 Diode Array Detector (Without Flow Cell) |
5082.0010 |
| Accessories |
|---|
| Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 13 µL Volume, 10mm Path Length |
6082.0100 |
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