The UltiMate® 3000 Binary Analytical system has been carefully configured to meet the demands of conventional HPLC and UHPLC at pressures up to 620 bar. Two serial dual-piston pumps provide stable, accurate pulse-free flows—essential for optimal binary gradient mixing performance—for consistent gradient formation, rapid gradient response, and excellent retention time precision.
- UHPLC compatibility with pressures up to 62 MPa (9000 psi)
- SmartFlow® technology for precise and accurate flow rates from 0.1 to 10 mL/min
- High-pressure gradient proportioning at superior accuracy and precision
- Minimized gradient delay volume for rapid gradient response
- High temperature operation for lower viscosity
- Ultralow-carryover, high-precision auto-injections
- Also suitable for fast LC applications (in combination with the RSLC conversion kit)
The UltiMate 3000 Binary Analytical system provides fast cycle time, reliable mobile phase temperature conditioning, and high data rate detection to ensure optimal performance for the full range of binary analytical applications.
| PUMP |
|---|
| Recommended pump | HPG-3200SD or HPG-3400SD |
Flow range (settable
) | 0.1–10 mL/min (0.001–10 mL/min) |
| Flow accuracy | ±0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD at 1 mL/min, whichever is greater |
| Pressure range | 2–62 MPa (290–9000 psi) |
| No. of eluent lines | 2 or 4 |
| Gradient delay volume | 400 µL |
| Solvent degassing | External (optional) |
| AUTOSAMPLER |
|---|
| Recommended autosampler | WPS-3000SL analytical |
| Injection volume range (recommended) | 0.01–100 µL (1–100 µL) Optional: 0.2–25 µL, 1.5–250 µL, 1.5–500 µL |
| Injection volume accuracy | ±0.5% at 50 and 90 µL |
| Injection volume precision | <0.25% RSD at 5 µL (typically <0.15% RSD), caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine in water |
| Carryover | <0.004% for caffeine with external wash at 75 bar |
| Injection cycle time | <15 s for 5 µL |
| Optional thermostating | 4–45 °C, at least 22 °C below ambient |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000SD |
| No. of switching valves | Optional, up to 2 |
| Recommended eluent preheater volume | 7 µL (0.01" i.d.) |
| Temperature range | 5 °C to 80 °C (max. 18 °C below ambient) |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| Heat-up/cool-down time | Typically: 15 min from 20 °C to 50 °C/ 15 min from 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended detector | MWD-3000 |
| Detector type | Multiple Wavelength |
| Number of channels | 8 |
| Wavelength range | 190–800 nm |
| Max. data collection rate | 100 Hz |
| Recommended flow cell | Analytical, 13 µL, 10 mm path |
| Noise | <±8 µAU at 254 nm (wide slit) |
| Drift | <1 mAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following modules and accessories represent a typical UltiMate 3000 Binary Analytical system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| SRD-3200 UltiMate 3000 Integrated Solvent and Degasser Rack, 2 Channels |
5035.9250 |
| HPG-3200SD - UltiMate 3000 Binary Analytical Pump |
5040.0021 |
| WPS-3000TSL UltiMate 3000 Thermostatted Analytical Sampler |
5822.0020 |
| TCC-3000SD Thermostatted Column Compartment |
5730.0010 |
| MWD-3000 - UltiMate 3000 Multiple Wavelength Detector (Without Flow Cell) |
5082.0030 |
| Accessories |
|---|
| Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 13 µL Volume, 10mm Path Length |
6082.0100 |
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