The UltiMate® 3000 Binary Semipreparative system is tailored for robust, safe, and high-performance purifications. Based on proven high-pressure gradient principles, the system takes full advantage of UltiMate 3000 features such as SmartFlow pulse-free eluent delivery and wide linear range detection.
- Wide operating flow rate range from 0.5 to 50 mL/min
- SmartFlow™ technology for precise and accurate flow rates
- High-pressure proportioning at high accuracy and precision
- Variable mixing volumes for different flow and delay volume requirements
- Sample injections up to 2.5 mL with excellent volumetric linearity
- Chromeleon® solvent and waste level monitor
The UltiMate 3000 Binary Semipreparative system supports high flow rates at low backpressure, thanks to the superior fluidic design of the semipreparative autosampler. The system is a robust platform for your purification of natural product extracts, isolation of impurities for structural elucidation, or other large-scale LC processes.
| Binary Semipreparative |
|---|
| Maximum system pressure | 10 MPa (1450 psi) |
| PUMP |
|---|
| Recommended pump | HPG-3200P |
| Settable flow range | 0–50 mL/min
(0.5–50 mL/min) |
| Flow accuracy | ±0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure range | 1–10 MPa (145–1450 psi) |
| No. of eluent lines | 2 |
| Gradient delay volume | 1035 µL |
| Biocompatible version | N/A |
| Solvent degassing | N/A |
| AUTOSAMPLER |
|---|
| Recommended sampler | WPS-3000SL Semiprep |
| Injection volume range | 0.01–2500 µL
(recommended.: 100–2500 µL) |
| Injection volume accuracy | ±1% at 2000 µL |
| Injection volume precision | <0.3% RSD at 100 µL
(typically <0.15% RSD),
caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <1% at 100–2000 µL, caffeine in water. |
| Carry over | <0.005% for caffeine with external wash at 75 bar |
| Injection cycle time | <20 s for 100 µL |
| Biocompatible version | N/A |
| Sample thermostating | With WPS-3000TSL, Semiprep: 4–45 °C, at least 22 °C below ambient |
| DETECTOR |
|---|
| Recommended detector | VWD-3100 |
| Detector type | Variable Wavelength |
| Number of channels | 1 |
| Wavelength range | 190–900 nm |
| Max. data collection rate | 20 Hz |
| Recommened flow cell | Semipreparative: 0.7 µL,
0.4 mm path |
| Noise | <±3.5 µAU at 254 nm |
| Drift | <0.1 mAU/h |
Performance Examples
The UltiMate® 3000 Intelligent LC series delivers improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates including preparative, analytic, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance.
The following modules and accessories represent a typical UltiMate 3000 Binary Semipreparative system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| HPG-3200P UltiMate 3000 Binary High-Pressure Proportioning Semi-Preparative Pump System |
5035.0025 |
| WPS-3000TSL UltiMate 3000 Thermostatted Semipreparative Autosampler |
5822.0028 |
| VWD-3100 20 Hz Single Channel Variable Wavelength Detector Without Flowcell |
5074.0005 |
| Accessories |
|---|
| Semi-preparative Flow Cell for VWD-3000 Series, PEEK, 0.7 µL Volume, 0.4 mm Pathlength |
6074.0320 |
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