The UltiMate® 3000 x2 Dual Rapid Separation LC (RSLC) system combines the ultrahigh speed and resolution of RSLC with x2 dual technology. Advanced chromatographic techniques such as parallel, tandem, and two-dimensional LC become a realistic and practical option, even at ultrahigh speed. Operate conventional or ultrahigh speed applications, increase sample throughput, achieve higher chromatographic resolutions, or automate tedious sample preparation steps such as analyte enrichment or matrix elimination. The system is ideal for ultrahigh resolution and conventional columns ranging from sub-2 μm to 10 µm particle size. The optimized system dimensions support any analytical HPLC column format and Dionex’s proprietary mixing concept easily adapts to the mixing volume required. Further RSLC system highlights include:
- Industry-leading flow-pressure footprint with flow rates up to 8 mL/min and pressure rating up to 15,000 psi (103.4 MPa)
- Zero-void-volume column and module connections using Viper™ fingertight fittings
- In-line split-loop injections for 15 second injections with no sample loss
- Up to two integrated ultrahigh-pressure valves, 6- or 10-port, 2-position or 7-port, 6-position
- Reduced backpressure with high-temperature column compartment operating up to 110 °C
- High data collection rate UV detectors (200 Hz), with variable wavelength, multiple wavelength, or diode array (with full spectral scan)
- Inject up to 500 µL even at 15,000 psi (103.4 MPa) system pressure with the patent pending injection valve of the rapid separation autosampler
| PUMP |
|---|
| Recommended pump | DGP-3600RS |
Flow range (settable) | 0.05–8 mL/min (0.001–8 mL/min ) |
| Flow accuracy | ± 0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure range | 2-103.4 MPa (15,000 psi) up to 5 mL/min, 2-80 MPa (11,600 psi) up to 8 mL/min |
| No of eluent lines | 6 (2 x 3) |
| Gradient delay volume | 690 µL by default (325–1790 µL with optional mixer kits), independent of operating pressure |
| Solvent degassing | External (optional) |
| AUTOSAMPLER |
|---|
| Recommended sampler | WPS-3000(T)RS |
| Injection volume range(recommended) | 0.01–100 µL (1–100 µL) |
| Injection volume accuracy | ±0.5% at 20 µL |
| Injection volume precision | <0.25% RSD at 5 µL (typical <0.15% RSD) caffeine in water |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–90µL, caffeine |
| Carryover | <0.005% for caffeine with external wash at 200 bar |
| Injection cycle time | <15 s for 5 µL |
| Sample thermostatting | 4–45°C, or 22°C below ambient (thermostatted sampler versions) |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000RS |
| No. of switching valves | Optional, up to 2 |
| Recommended eluent pre-heater volume | 2 µL (0.005" i.d.) |
| Temperature range | 5 °C to 110 °C (min. 18 °C below ambient) |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| Heat-up/cool-down time | Typical: 12 min from 20 °C to 50 °C/ 15 min form 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended detector | DAD-3000RS |
| Detector type | Diode wavelength |
| Number of channels | 8 plus 3D field |
| Wavelength range | 190–800 nm |
| Max. data collection rate | 200 Hz (including 3D field) |
| Recommended Flow Cell | Semi-micro, 2.5 µL, 7 mm path Analytical, 13 µL, 10 mm path |
| Noise | < ±8 µAU at 254 nm (wide slit) |
| Drift | <0.1 µAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.