The UltiMate^® 3000 RSLCnano LC system offers better confidence; confidence in performance, confidence in results, and confidence in ease of operation. Dionex’s 20 year legacy in nano LC has resulted in a system that provides better results than ever before, setting a new standard in ease-of-use and performance for nano LC.

The heart of the UltiMate 3000 RSLCnano system is the continuous direct flow delivery. The HPG nano pump together with the innovative flow meter generates a continuous, pulse-free, and direct flow. Refill cycles are not required, therefore the system provides uninterrupted operation as long as there is mobile phase available. The unmatched flow-pressure footprint of 800 bar column pressure is available from 20 nL/min to 50 µL/min and offers the pump power to drive any separation.

The built-in column compartment features up to two micro switching valves capable of working at pressures up to 1000 bar. The valves can be pulled forward or taken out without tools, to maximize ease-of-use when setting up a new application.

Tedious and troublesome nano LC application configuration is a thing of the past with the new nanoViper^™ capillary fitting system. nanoViper brings unparalleled ease-of-use to nano LC, providing fingertight, zero-dead-volume connections to any module, valve, and column over the complete pressure range.

The RSLCnano system is more than just a splitless nano instrument. The system is designed with application flexibility in mind. The RSLCnano system can be operated at nano, capillary, and micro flow rates, configured for a large range of 1D and 2D workflows, and can be operated tool-free using nanoViper connections.

This makes the RSLCnano system the ideal high-resolution LC front end to any MS system.

Comparisons

The UltiMate^® 3000 RSLCnano system, a Rapid Separation LC system, features the widest flow-pressure footprint in the market today. It allows nano, capillary, and micro flow rates at column pressures up to 800 bars. This flow-pressure footprint is unmatched by current nano LC offerings from other vendors.

In the image below the flow pressure footprint of the RSLCnano is compared to other nano LC vendors

Even when compared to vendors offering UHPLC nano systems, the flow-pressure footprint of the RSLCnano remains unmatched.

Only this large flow-pressure footprint offers the application flexibility required by analytical laboratories and enables use of the full potential of Acclaim^® PepMap^™ RSLC columns.

Unparalleled Retention Time Precision in Nano LC

As with any chromatographic technique, retention time precision is of utmost importance in nano LC. Reproducible chromatography will help data processing and sample comparison in quantitative proteomics by allowing the application of a smaller retention time deviation threshold. The new UltiMate^® 3000 RSLCnano system was designed to deliver reliable, high precision flow rates. In a recent experiment at our demo labs, the RSLCnano showed excellent retention time performance with an average RSD value as low as 0.03% for all peptides, illustrated in the chromatogram overlay below.

Overlay of nine consecutive peptide separations obtained with the UltiMate 3000 RSLCnano in preconcentration mode.

Conditions: UltiMate 3000 RSLCnano system, 75 µm i.d. x 15 cm Acclaim^® PepMap™ RSLC column (C18, 2 µm), flow rate of 1.1 µL/min, detection UV at 214 nm, and sample: six protein digest (PMD).