The UltiMate® 3000 Quaternary Rapid Separation LC (RSLC) system is the ideal combination of quaternary eluent proportioning and RSLC technology. Use up to four solvents at up to 15,000 psi (103.4 MPa) for fast routine applications, and method development. The system is ideal for ultrahigh resolution and conventional columns with particles ranging from sub-2 μm to 10 µm particle size. The optimized system dimensions support any analytical HPLC column format and Dionex’s proprietary mixing concept easily adapts to the mixing volume required. Further RSLC system highlights include:
- Industry leading flow-pressure footprint with flow rates up to 8 mL/min and pressure up to 15,000 psi
(103.4 MPa). - Zero-void-volume module connections using Viper™ fingertight fittings
- In-line split-loop injections for 15 second, no-sample loss injections
- Reduced backpressure with high temperature column compartment operating up to 110 °C
- Various detectors with up to 200 Hz data collection rate, including variable wavelength, multiple wavelength, and diode array (with full spectral scan) UV detectors, fluorescence detectors, and charged aerosol detectors
- Inject up to 500 µL even at maximum system pressure with the patented injection valve of the Rapid Separation Autosampler
| PUMP |
|---|
| Recommended pump | LPG-3400RS |
| Flow range (settable range) | 0.1–8 mL/min (0.001–8 mL/min) |
| Flow accuracy | ±0.1% |
| Flow precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure Range | 2-103.4 MPa (15,000 psi) up to 5 mL/min, 2-80 MPa (11,600 psi) up to 8 mL/min |
| No of Eluent lines | 4 |
| Gradient delay volume | 690 μL (325–1790 μL with optional mixer kits) |
| Solvent degassing | Built-in, 4-channels |
| AUTOSAMPLER |
|---|
| Recommendation sampler | WPS-3000(T)RS |
| Injection volume range(recommended) | 0.01–100 µL (1–100 µL) Optional: 0.2–25 µL, 1.5–250 µL, 1.5–500 µL |
| Injection volume accuracy | ±0.5% at 20 µL |
| Injection volume precision | <0.25% RSD at 5 µL (typical <0.15% RSD), caffeine in water |
| Linearity | Corr. coeff. > 0.9999, RSD <0.5% at 5–90µL, caffeine |
| Carry over | <0.005% for caffeine with external wash at 200 bar |
| Injection cycle time | <15 s for 5 µL |
| Sample Thermostatting | 4–45 °C, or 22 °C below ambient (thermostatted sampler versions) |
| COLUMN COMPARTMENT |
|---|
| Recommended column compartment | TCC-3000RS |
| No. of switching valves | Optional, up to 2 |
| Recommended eluent pre-heater volume | 2 µL (0.005" i.d.) |
| Temperature range | 5 °C–110 °C (min. 18 °C below ambient) |
| Temperature accuracy | ±0.5 °C |
| Temperature stability/precision | ±0.1 °C |
| Heat-Up/cool-down time | Typical: 15 min from 20 °C to 50 °C/ 15 min form 50 °C to 20 °C |
| DETECTOR |
|---|
| Recommended detector | VWD-3400RS |
| Detector Type | Variable Wavelength |
| Number of Channels | 4 |
| Wavelength range | 190–900 nm |
| Max. data collection rate | 200 Hz (single channel) |
| Recommended flow cell | Semi-micro, 2.5 µL, 7 mm path |
| Noise | < ±3.5 µAU at 254 nm |
| Drift | <0.1 µAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
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