The UltiMate^® 3000 Binary Rapid Separation LC (RSLC) system is the perfect choice for developing the fastest UHPLC separations possible. The binary RSLC pump features the smallest gradient-delay volume of the Dionex RSLC family of pumps and provides smooth, flawless eluent delivery with even the smallest volume column. The system’s powerful combination of high flow rate capability, high pressure rating, and ultrafast data collection rate facilitates the highest peak capacity in the shortest run times. Even separations of 10 peaks in 10 seconds are feasible. But the system is not only designed for ultrafast separations. The optimized system dimensions support any analytical HPLC column format and Dionex’s proprietary mixing concept easily adapts to any mixing volume required. Further RSLC system highlights include:

• Industry leading flow-pressure footprint with flow rates up to 8 mL/min and pressure rating up to 15,000 psi (103.4 MPa).
• Zero-dead-volume module connections using Viper^™ capillary and fitting technology
• In-line split-loop injections for 15 second sample delivery with no sample loss
• Reduced system backpressure with high-temperature column compartment operating up to 110 °C
• Data collection rates up to 200 Hz with a wide range of optical detectors including three UV variants and fluorescence
• Inject up to 500 µL at high system pressure (up to15,000 psi (103.4 MPa)) using the patented injection valve of the Rapid Separation Autosampler

PUMP
Recommended pump	HPG-3200RS or HPG-3400RS
Flow range (settable )	0.05–8 mL/min (0.001–8 mL/min)
Flow accuracy	± 0.1%
Flow precision	< 0.05% RSD or <0.01 min SD, whichever is greater
Pressure range	2-103.4 MPa (15,000 psi) up to 5 mL/min, 2-80 MPa (11,600 psi) up to 8 mL/min
No of eluent lines	2 or 4
Gradient delay volume	200 µL  (35–1500 µL with optional mixer kits)
Solvent degassing	External (optional)

AUTOSAMPLER
Recommendation sampler	WPS-3000(T)RS
Injection volume range(recommended)	0.01–100 µL  (1–100 µL) Optional: 0.2–25 µL, 1.5–250 µL, 1.5–500 µL
Injection volume accuracy	±0.5% at 20 µL
Injection volume precision	<0.25% RSD at 5 µL (typical <0.15% RSD), caffeine in water
Linearity	Corr. coeff. >0.9999, RSD <0.5% at 5–90 µL, caffeine in water
Carryover	<0.004% for caffeine with external wash at 200 bar
Injection cycle time	<15 s for 5 µL
Sample thermostatting	4–45 °C, or 22 °C below ambient (thermostatted sampler versions)

COLUMN COMPARTMENT
Recommended column compartment	TCC-3000RS
No. of switching valves	Optional, up to 2
Recommended eluent pre-heater volume	2 µL (0.005" i.d.)
Temperature range	5 °C–110 °C (min. 18 °C below ambient)
Temperature accuracy	±0.5 °C
Temperature stability/precision	±0.1 °C
Heat-up/cool-down time	Typically: 12 min from 20 °C to 50 °C/ 15 min from 50 °C to 20 °C

DETECTOR
Recommended detector	DAD-3000RS
Detector type	Diode Array
Number of channels	8 + 3D field
Wavelength range	190–800 nm
Max. data collection rate	200 Hz (including 3D filed)
Recommended flow cell	Semi-micro, 2.5 µL, 7 mm path
Noise	<±8 µAU at 254 nm (wide slit)
Drift	<1 mAU/h (typically <0.5 mAU/h)

UltiMate 3000 RSLC Systems
Conventional and UHPLC Separations
Robust Viper Connections
Outstanding Precision with the UltiMate 3000 RSLC System
Efficiently Accelerate Existing HPLC Methods with RSLC
10 Peaks in 10 Seconds
Almost Zero Autosampler Carry-Over
Short UltiMate 3000 Cycle Times for Maximum Throughput
Constant and Independent Column Temperature Control
Efficient Precolumn Heating
Precise Peak Identification with RSLC Methods
Faster Results in Chemical Process Control
Instant Process Control Results
Fast Separation of Eight Softdrink Additives
Ultra-High Resolution Analysis of 29 Pesticides
Reduction of Eluent Consumption
Speed-up of Complex Separations
Ultrafast Amino Acid Analysis
Fast Amino Acid Analysis on a Sub-2 µm Column

Performance
Enhanced Isocratic Retention Time Precision
Enhanced Gradient Retention Time Precision
Reduced Total Cycle Time
Enhanced Autosampler Injection Volume Precision
Enhanced Impurity Quantification
Reduced Detector Noise and Drift
Enhanced Detector Linearity
Enhanced Autosampler Linearity
Enhanced Autosampler Temperature Accuracy
Enhanced Autosampler Injection Volume Accuracy

Reliability
Enhanced System to System Reproducibility
Enhanced Column Compartment Temperature Stability

Product Brochures
	UltiMate 3000 RSLC System Brochure
	UltiMate 3000 Liquid Chromatography Systems Brochure

Technical Notes
	TN 75: Easy Method Transfer from HPLC to RSLC with the Dionex Method Speed-Up Calculator
	TN 88: UHPLC Method Transfer at Its Best—A Feature Update of the Dionex RSLC Method Transfer Calculator

Posters
	Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control

The following RSLC modules and accessories configure the most flexible RSLC system.

Hardware
SRD-3400 UltiMate 3000 Integrated Solvent and Degasser Rack, 4 Channels	5035.9245
HPG-3400RS UltiMate 3000 Binary Rapid Separation Pump with Solvent Selector Valves	5040.0046
WPS-3000TRS Rapid Separation Wellplate Sampler, Thermostatted	5840.0020
TCC-3000RS - UltiMate 3000 Rapid Separation Thermostatted Column Compartment	5730.0000
DAD-3000RS - UltiMate 3000 Rapid Separation Diode Array Detector (Without Flow Cell)	5082.0020

Problem assembling output for item: 1-101-73927 with template: dxpSnTableRowPart exception: Index: 0, Size: 0 see log for stack trace

Accessories
Sample loop, 25 μL, for UltiMate 3000 WPS-3000SL Analytical and WPS-3000RS Autosampler Series, until S/N 8049346	6820.2415
Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 13 µL Volume, 10mm Path Length	6082.0100
Semi-Micro Flow Cell for DAD-3000 and MWD-3000 Series, SST, 2.5 µL Volume, 7 mm Path Length	6082.0300