The Basic Automated LC system is a modular system optimized for reliability and ease-of-use with routine LC applications. Typically configured with the ISO-3100SD Isocratic Analytical Pump or the LPG-3400SD Quaternary Analytical Pump, the system easily handles conventional HPLC and also provides cost-effective access to UHPLC at up to 62 MPa (9000 psi). The heart of this system is the ACC-3000 Autosampler Column Compartment, a combined autosampler and column compartment, for cost-conscious automation and the best available value for your budget.
- Excellent retention time and gradient precision due to SmartFlow® technology
- Pulled-loop injection principle (full- and partial-loop injections)
- Fast and stable column thermostatting between 5 °C above ambient and 50 °C
- High detection sensitivity with low detector noise and drift
- Robust, dependable performance at low cost of ownership
| PUMPS |
|---|
| Recommended Pump | LPG-3400SD |
Flow Range
(Settable Range) | 0.2–10 mL/min
(0.001–10 mL/min) |
| Flow Accuracy | ±0.1% |
| Flow Precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure Range | 2–62 MPa (290–9000 psi) |
| No. of Eluent Lines | 4 |
| Gradient Delay Volume | 690 µL by default (325–1790 µL with optional mixer kits), independent of operating pressure |
| Solvent Degassing | Built-in, 4 channels |
| AUTOSAMPLER |
|---|
| Recommended Sampler | ACC-3000 |
| Injection Volume Range | 1–10 (partial loop) or
20 µL (full loop), up to 200 µL (optional) |
| Injection Volume Accuracy | N/A |
| Injection Volume Precision | <0.25% RSD in full-loop mode (20 µL loop)typically <0.3% RSD in partial-loop mode at 5 µL (20 µL loop) |
| Linearity | Corr. coeff. >0.9995, RSD <1% partial loop for 1–10 µL (20 µL loop) |
| Carryover | <0.02% for caffeine with external wash |
| Sample thermostating | With ACC-3000T: 8–40 °C, min. 15 °C below ambient |
| COLUMN COMPARTMENT |
|---|
| Recommended Column Compartment/Flow Manager | Integrated with ACC-3000 |
| No. of Switching Valves | N/A |
Recommened Eluent
Pre-Heater Volume | Column inlet tubing |
| Temperature Range | 5 °C above ambient to 50 °C |
| DETECTOR |
|---|
| Recommended Detectors | VWD-3100 |
| Detector Type | Variable Wavelength UV |
| Number of Channels | 1 |
| Wavelength Range | 190–900 nm |
| Max. Data Collection Rate | 100 Hz |
| Recommended Flow Cell | Analytical: 11 µL, 10 mm path |
| Noise | <±3.5 µAU at 254 nm |
| Drift | <0.1 µAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
The following LC modules and accessories represent a typical UltiMate 3000 Basic Automated system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| SR-3000 UltiMate 3000 Solvent Rack without Degasser |
5035.9200 |
| ISO-3100SD - UltiMate 3000 Isocratic Analytical Pump |
5040.0011 |
| LPG-3400SD - UltiMate 3000 Quaternary Analytical Pump |
5040.0031 |
| ACC-3000T UltiMate 3000 Thermostatted Autosampler with Integrated Column Compartment |
5830.0020 |
| VWD-3100 - UltiMate 3000 Single Channel Variable Wavelength Detector (Without Flow Cell) |
5074.0005 |
| Accessories |
|---|
| Analytical Flow Cell for VWD-3000 Series, SST, 11 µL Volume, 10 mm Pathlength |
6074.0250 |
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