Dionex Corporation Home

Rapid Separation LC

The UltiMate® 3000 LCi Solution for Rapid Separation Liquid Chromatography (RSLC) provides the means for ultrafast, high resolution chromatography: Achieve ultrahigh performance chromatography (UHPLC) separations at a maximum level of reliability and flexibility.

  • Accelerate your analytical LC application by up to a factor of 50
  • Accelerate and revalidate existing LC methods in less than one week
  • Improve resolution for the analysis of complex samples by increasing peak capacity
  • Increase flexibility with the Rapid Separation LC System in combination with Acclaim 1, 2.1, 3 and 4.6 mm ID columns with particle sizes of 2, 3 and 5 μm
  • Enjoy Chromeleon® data processing and reporting tools, including 3-D data handling and revalidation tools for instant results

The extensive flow-pressure footprint of the UltiMate 3000 Rapid Separation LC system and the comprehensive set of Acclaim column formats allow you to exploit the full range of LC applications at flow rates up to 5 mL/min and backpressures up to 800 bar.

With its extensive flow-pressure footprint, RSLC fully meets your chromatographic goals. Simply work with your column of choice and in the appropriate zone for your application (Zone 1: Ultrahigh resolution/high speed, Zone 2: Conventional LC, Zone 3: Very high speed/very high resolution, Zone 4: Ultrahigh speed/ high resolution). RSLC covers them all.

A method speed-up calculator is provided for quick and easy conversion of conventional LC to RSLC methods.

The Dionex Method Speed-Up Calculator facilitates the process of method adaptation to fast LC. Enter your old method details and your new column details. The calculator immediately provides new method parameters and automatically calculates how much time and solvent the new method will save.

The UltiMate 3000 LCi Solution for RSLC significantly increases sample throughput of LC applications such as amino acid analysis, without compromising resolution.

Accelerate your amino acid analysis: Conventional reversed-phase LC separation of 21 amino acids in 41 min (60 min total run time, left chromatogram) vs. baseline separation of 21 amino acids in less than 6 min (7 min total run time, right chromatogram) on the Acclaim RSLC 2.2 μm column.

UltiMate 3000 systems are available in three system configurations, the Binary RSLC System, the Quaternary RSLC System, and the x2 Dual RSLC System.