The UltiMate® 3000 Binary Rapid Separation LC Pump HPG-3x00RS operates at ultrahigh backpressures and high flow rates simultaneously. Achieve the highest separation efficiency in combination with long, small bore ≤ 2 μm columns, or use with conventional LC columns: The RS pump is the most flexible binary UHPLC pump available.
- Serial dual-piston operating principle, high-pressure gradient proportioning
- Wide operating flow rate range from 50 μL/min to 8 mL/min with high flow rate accuracy
- Support of operating pressures of up to 100 MPa (15,000 psi)
- SmartFlow® technology for high flow rate, gradient, and retention time precision
- Perfectly balance gradient delay volume against mobile phase mixing efficiency
- Optional solvent selection valves support up to four different mobile phases
- Active rear-seal wash and floating pistons for maximum seal lifetime
The HPG-3x00RS is ideal for MS front-end configurations, microflow, analytical, and ultrafast, high-resolution separations in pharmaceutical, life science, and environmental laboratories. The gradient delay volume can be adjusted easily according to your requirements (e.g., 35 μL for MS front end and 400 μL for analytical LC) for optimal mixing efficiency.
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
Optimum Mixing of Solvents with the UltiMate RSLC Pump
Ultrafast gradient separations require a small gradient delay volume to ensure the gradient is correctly applied to the column. However, high mixing volumes are typically required to ensure the mobile phase components are completely mixed.This is particularly important when working with UV detectors, as incomplete mixing may lead to ripple effects in the chromatography baseline.
With very fast separations even small fluctuations in the mobile phase composition can compromise the precision of the peak retention time. Therefore, complete mixing is necessary to achieve a constant, flat baseline, high signal-to-noise ratios, and accurate peak identification.
Different types of applications have different requirements regarding mixing efficiency. Some require almost no mixing, while others require additional mixing volume to achieve a smooth baseline. A water/acetonitrile gradient using TFA as a modifier is an example of this type of gradient that requires precise mixing efficiency
The new proprietary mixing concept from Dionex addresses all these needs with:
- A two-step process of radial mixing followed by longitudinal mixing to ensure the highest possible mixing efficiency.
- Mixing volume that can be easily adapted to fit specific method requirements. The default mixing volume (400 µL) can be easily changed using mixing kits of 35, 200, 800, or 1500 µL (effective mixing volume).
- Available for the UltiMate® 3000 Binary, Quaternary, and Dual-Gradient RSLC pumps providing ultrahigh resolution and ultrafast separations.
| Binary Rapid Separation Pump Specifications |
|---|
| Operating Principle | Serial dual-piston |
| Flow Rate Range | 0.001–8 mL/min (recommended: 0.05–8 mL/min) |
| Flow Accuracy | ±0.1% |
| Flow Precision | < 0.05% RSD or < 0.01 min SD, whichever is greater |
| Pressure Range | 2–100 MPa (15,000 psi) up to 5 mL/min, 2–80 MPa (11,600 psi) up to 8 mL/min |
| Pulsation | Typically: < 2 bar or < 1% whichever is greater |
| Gradient Formation | High-pressure proportioning |
| Proportioning Accuracy | ±0.2% (of full scale) |
| Proportioning Precision | < 0.15% SD |
| Number of Eluent Lines | 2 |
| Gradient Delay Volume | 200 µL
(35–1,500 μL with optional mixer kits)
independent of operating pressure |
| Version with Solvent Selector Valve | HPG-3400RS |
| Solvent Degassing | External (optional) |
| Dimensions (h × w × d) | 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.) |
| GLP Features | Full support of Automatic Equipment Qualification (AutoQ™), Qualification Status and System Wellness Monitoring.
All system parameters are logged in the Chromeleon® software Audit Trail. |
| I/O Interfaces | 2 digital inputs, 2 relay outputs |
| Communications | USB for PC connection;
USB hub with 3 sockets integrated;
15-pin D-Sub connector for solvent rack/degasser connection. |
| Hardware |
|---|
| HPG-3200RS UltiMate 3000 Binary Rapid Separation Pump |
5040.0026 |
| SRD-3200 UltiMate 3000 Integrated Solvent and Degasser Rack, 2 Channels |
5035.9250 |
| HPG-3400RS UltiMate 3000 Binary Rapid Separation Pump with Solvent Selector Valves |
5040.0046 |
| SRD-3400 UltiMate 3000 Integrated Solvent and Degasser Rack, 4 Channels |
5035.9245 |
| Accessories |
|---|
| Static Mixer Kit, Micro, 35 µL Mixing Volume |
6040.5000 |
| Mixer Kit with 200 µL Mixing Volume for UltiMate 3000 RS/SD Pumps
|
6040.5151 |
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