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Probot MALDI Spotter
Probot MALDI Spotter
The Probot MALDI spotter is designed for on-line collection of nL- and μL-fractions eluting from nano and capillary LC systems on different types of sample carriers such as MALDI targets, PVDF membranes, 96, 384, or 1536 well plates, or microchip structures. The X,Y,Z-moving table and fixed needle design allow nL-spotting with excellent precision and minimal dead volume.
- Robotic system for MALDI spotting (and fraction collection)
- Supports MALDI targets and sample carriers from every vendor
- Small nL-volumes collection with zero chromatographic dispersion maintaining LC resolution
- Two well plate (or six AB4700) capacity for collection of more than 4000 spots
- Integrated reagent (matrix) addition for easy MALDI spotting
- Dual-collection mode for fraction splitting over two different sample carriers
Typical Probot applications include on-line MALDI target preparation or microfraction collection. Due to its accuracy and flexibility, the Probot is the ideal nano-LC/MALDI-MS interface, integrating the high-sensitivity separation power of nano-LC and high resolving power of MALDI-MS.
| Microfraction Collector Specifications |
|---|
| Table Dimensions (w × d) | 210 × 135 mm or 8.27 × 5.31 in. |
| Table Movement | X = 195 mm or 7.68 in.; speed 0.250–20 mm/s Y = 130 mm or 5.12 in.; speed 0.125–10 mm/s Z = 95 mm or 3.74 in.; speed 0.125–10 mm/s |
| Precision of Table | Better than 20 µm |
| Spotting Frequency | User-selectable down to 5 s with minimal volumes of 5nL |
| Lifting Force | >2 kp |
| Reference Position | All axes are controlled by reference switches Reproducibility is ± 1 motor step (2.5 µm) |
| Carryover | In pipetting mode, less than 0.02% (with additional needle wash) |
| Safety | All functions are stopped when: - The front door is opened - An axis runs over its end switch - The table exerts too much force on the needle In CZE mode, the CZE voltage is interrupted when: - The front cover is opened - Probot is unplugged or switched off |
| Connections | Remote, X, Y, Z RS-232 communication Remote start input and TTL input Outputs: Four relay outputs (optional) HV switch off (safety feature for CZE) |
| Dimensions (h × w × d) | 30 × 48 × 50 cm (12 × 19 × 20 in.) |
| Weight | 23 kg (51 lb) |
| Software | Fully controlled by microcarrier |
| Collection Modes | Time-based collection and peak collection |
| Hardware |
| Probot Microfraction Collector with Dosage Unit |
5713.0200 |
| Accessories |
| Universal Table for ABI 4700 Proteomics Analyzer for 6 Targets |
5713.6402 |
| Probot Table Adapter for AB/MDS-Sciex ABI 4800 |
6713.0022 |
| Probot Table Adapter for 2 x Bruker MALDI Target (Micro Titer Plate Type) |
5713.6307 |
| Probot Table Adapter for 6 Micromass Rectangular Targets |
5713.6328 |
| Probot Table Adapter for 2 x Shimadzu/Kratos Axima QIT Targets |
5713.6319 |
| Probot Table Adapter for Thermo Scientic vMaldi |
6713.0025 |
| Probot Demonstration Table Adapter with Adjustable Strips |
5713.6338 |
Problem assembling output for item: 1-101-74040 with template: dxpSnTableRowPart exception: Index: 0, Size: 0 see log for stack trace
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