Home >
Dionex Products >
Liquid Chromatography >
LC Modules >
LC Detectors >
Fluorescence Detector
Fluorescence Detector
The FLD-3000 is a high-sensitivity fluorescence detector series for the UltiMate® 3000 HPLC systems. It is available in Rapid Separation (200 Hz) and Standard (100 Hz) versions. The optics of the FLD-3000 Series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, or emisssion scans, zero order mode, or multichannel acquisition. Operated with the Chromeleon® Chromatography Data System software, the detector provides automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.
- Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations.
- Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference).
- Unsurpassed reproducibility with active flow cell temperature-control for stable fluorophore activity independent from changes in ambient temperature.
- Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing.
- Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range.
- 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization.
- Innovative Variable Emission Filter for real-time compound-related sensitivity optimization (FLD-3400RS only).
- Large front-panel display for easy monitoring of the detector status.
- Flow cell syringe injection kit as a cost-effective solution to perform an off-line measurement.
- Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns.
- Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells.
| Fluorescence Detector Specifications |
|---|
| Light Source | Xenon Flash Lamp |
| Lamp Pulse Frequency: | HighPower (~300 Hz) Standard (~100 Hz) LongLife (~20 Hz) |
| Data Collection Rate: | Single wavelength: up to 200 Hz (FLD-3400RS) Multiple wavelength: up to 4 Hz (FLD-3400RS) Single wavelength: up to 100 Hz (FLD-3100) |
| Spectrum Scanning Modes: | 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control) |
| Wavelength Range: | | Variant | Ex Min | Ex Max | Em Min | Em Max | | 3100 | 200 | 630 | 265 | 650 | | 3400RS | 200 | 630 | 220 | 650 | | 3100 with Dual-PMT | 200 | 880 | 265 | 900 | | 3400RS with Dual-PMT | 200 | 880 | 220 | 900 |
|
| Spectral Bandwidth: | Excitation: 20 nm Emission: 20 nm |
| Emission Filter: | Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100) |
| No. of Fluorescence Channels: | Up to 4 (FLD-3400RS) 1 (FLD-3100) |
| Excitation/Emission Wavelength Switching Time: | < 250 ms |
| Wavelength Accuracy: | ±2 nm |
| Wavelength Repeatability: | ±0.2 nm |
| Sensitivity: | Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference) |
| Flow Cell Thermostatting: | Ambient + 10 °C to 50 °C |
| Flow Cell: | Analytical flow cell volume: 8 μL 20 bar (290 psi) maximum cell pressure Micro flow cell volume: 2 μL 40 bar (580 psi) maximum cell pressure |
| PC Connection: | All functions controllable through USB |
| GLP Features: | Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip |
| Dimensions (h × w × d): | 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.) |
| Weight: | 18 kg |
| Hardware |
| FLD-3100 - UltiMate 3000 Fluorescence Detector (without Flow Cell) |
5078.0010 |
| FLD-3100 - UltiMate 3000 Fluorescence Detector with Dual-PMT (without Flow Cell) |
5078.0015 |
| FLD-3400RS - UltiMate 3000 Rapid Separation Fluorescence Detector (without Flow Cell) |
5078.0020 |
| FLD-3400RS - UltiMate 3000 Rapid Separation Fluorescence Detector with Dual-PMT (without Flow Cell) |
5078.0025 |
| Accessories |
| Analytical Flow Cell for FLD-3000 Series, SST, 8 µL Volume
|
6078.4230 |
| Micro Flow Cell for FLD-3000 Series, SST, 2 µL Volume
|
6078.4330 |
| Upgrade Option to Dual-PMT Version for FLD-3000 Series
|
6078.5360 |
| Flow Cell Syringe Injection and Flushing kit for FLD-3000 Series
|
6078.4200 |
- Chromatography Data Systems