Diode Array Detector
The Thermo Scientifc™ Dionex™ UltiMate™ 3000 Rapid Separation Diode Array Detector (DAD-3000RS) is the detector of choice if you need maximum flexibility in your laboratory. It offers reliable UV-vis detection for both conventional and rapid separation LC applications with full wavelength spectra acquisition, sub-1 nm pixel resolution, and data collection rates up to 200 Hz.
- Low noise (typically <±7 μAU) and drift (typically <0.5 mAU/h) for lowest LODs and LOQs
- Data collection rates of up to 200 Hz allow detection and characterization of even the sharpest peaks
- Full UV-vis spectra data acquisition even for 10 peaks separated in 10 s
- Stainless steel and PEEK™ flow cells provide maximum application flexibility
- Front access to prealigned cells and lamps simplifies routine maintenance
- ID chips for tracking of lamp and cell parameters in audit trails
The DAD-3000RS features eight channels of single-wavelength data acquisition, a 1024 element diode array, and excellent noise and drift performance close to forward-optic detectors. The comprehensive set of 3D data tools in the Thermo Scientifc™ Dionex™ Chromeleon™ Chromatography Data System software makes it easy to process and report your data.
The Thermo Scientific™ Dionex™ UltiMate™ 3000 PCM-3000 pH and Conductivity Monitor is an upgrade for accurate pH and conductivity on-line monitoring with the DAD-3000 Series. A typical application area of this unit is monitoring the gradient formation in salt- or pH-gradient-based ion-exchange chromatography (IEC) in biopharmaceutical analysis.
Note: A standard version, DAD-3000, with a data rate of up to 100 Hz is also available for use in combination with UltiMate 3000 analytical systems.
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
|Enhanced System to System Reproducibility|
|Enhanced Column Compartment Temperature Stability|
|Diode Array Detector Specifications|
|Optical System||Single-beam, reverse optics design with concave holographic grating|
|Maximum Data Collection Rate||200 Hz: DAD-3000RS (including 3-D acquisition) under Chromeleon 7.1 control|
100 Hz: DAD-3000 (including 3-D acquisition)
|Wavelength Range||190 to 800 nm|
|Noise||Wide slit: < ±8µAU at 254 nm|
Narrow slit< ±10 µAU at 254 nm
response time 2 s (according to ASTM time constant approx 1 s), 4 nm bandwidth, water at 1.0 mL/min
|Drift||<1 mAU/h (typically <0.5 mAU/h) at 254 nm and 520 nm with water at 1.0 mL/min|
|Linearity||<3% RSD and corr. coeff. >0.9995 up to 1.5 AU,|
typically <5% RSD and corr. coeff. ≥0.999 up to 2.0 AU
|Light Source||Deuterium lamp, tungsten lamp, temperature control for both lamps|
|Wavelength Accuracy||±1.0 nm, self-calibration with D-alpha line, verification with holmium oxide filter|
|Pixel resolution||<1 nm|
|Slit Width||Narrow or wide slit, settable for the DAD-3000RS|
|PC Connection||All functions controllable via USB 2.0; integrated USB hub with three USB 2.0 ports|
|GLP Features||Automatic Instrument Qualification (AutoQ™), System Wellness and Qualification Monitoring with Chromeleon® software, lamp and cell ID chips|
|User Input/Display||LCD indicating system paramenters, standby button,|
three LEDs for status monitoring,
four function keys for initial operation and maintenance
|Power Requirements||85–260 V, 50/60 Hz, max. 150 W|
|Dimensions (h × w × d)||16 × 42 × 51 cm (6.3 × 16.5 × 20 in.)|
The Diode Array Detector DAD-3000RS and DAD-3000 are shipped without flow cells. Flow cells must be ordered separately and according to your LC application requirements.
|DAD-3000RS - UltiMate 3000 Rapid Separation Diode Array Detector (Without Flow Cell)||5082.0020|
|DAD-3000 - UltiMate 3000 Diode Array Detector (Without Flow Cell)||5082.0010|
|Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 13 µL Volume, 10mm Path Length||6082.0100|
|Semi-Analytical Flow Cell for DAD-3000 and MWD-3000 Series, SST, 5 µL Volume, 7 mm Path Length||6082.0200|
|Semi-Micro Flow Cell for DAD-3000 and MWD-3000 Series, SST, 2.5 µL Volume, 7 mm Path Length||6082.0300|
|Analytical Flow Cell for DAD-3000 and MWD-3000 Series, PEEK, 13 µL Volume, 10mm Path Length||6082.0400|
|Semi-Micro Flow Cell for DAD-3000 and MWD-3000 Series, PEEK, 2.5 µL Volume, 7 mm Path Length||6082.0500|
|Semi-Preparative Flow Cell for DAD-3000 and MWD-3000 Series, PEEK, 0.7 µL Volume, 0.4 mm Path Length||6082.0600|
|PCM-3000 UltiMate 3000 pH and Conductivity Monitor for the use with DAD-3000 and MWD-3000||6082.2000|