The WPS-3000FC Autosampler supports sample injection and fractionation using two integrated switching valves. The instrument fully automates off-line multidimensional and multistep liquid chromatography in analytical and micro flow rate ranges. The WPS-3000FC simplifies multidimensional separations by allowing columns that differ in chemistry and internal diameter to be combined on a single LC system with dual-gradient flow delivery. The WPS-3000FC is available in stainless steel and biocompatible PEEK™ versions, making it an ideal tool for biomolecule analysis, including protein and antibody fractionation and purification. In combination with the Extended Fraction Collection capabilities of the Chromeleon® Chromatography Data System software, the WPS-3000FC enables full automation of injection, fractionation, and re-injection of samples.
Key features of the WPS-3000FC Autosampler:
- Reduced benchspace required
- Sample and fraction cooling
- Wide application flexibility, including automated off-line 2D-LC, multistep protein purification/analysis, sample derivatization, protein digestion, neutralization
- Three well plate capacity and up to 1100 sample and fraction positions
- Fractionation in sealed and capped sample containers
- Chromeleon Chromatography Data System software for advanced workflows
- Software templates for automated off-line 2D-LC

Automated monoclonal antibody purification on Protein A and isoform separation on a ProPac® WCX column.
PEEK is a trademark of Victrex PLC.
| Analytical Autosampler with Fraction Collector Specifications |
|---|
| Sample capacity | 120 × 1.8 mL/2.0 mL vials, 120 × 1.1 mL conical vials, 216 × 1.2 mL vials, 216 × 0.3 mL vials, 30 × 10 mL vials and/or 66 × 4 mL vials, 3 × 96 and/or 384 normal or deep well plates + 15 × 10 mL vials (depending on sample tray configuration) |
| Injection methods | Full-loop and partial-loop injections, low dispersion mode, microliter pickup, user-defined programs |
Injection volume range (recommended) | 0.01–250 μL (0.1–250 μL) |
| Minimum sample required | 1 μL out of 1 μL (microliter pickup) |
| Injection volume precision | <0.25% RSD at 5 μL in full-loop and (<0.3% RSD at 5 μL and 20 μL partial-loop mode, caffeine in water) |
| Linearity | Corr. coeff. >0.9999, RSD <0.5% at 5–30 μL, caffeine in water |
| Needle wash | Active external needle wash |
| Carryover | <0.02% for caffeine with external wash |
| Injection cycle time | <60 s for 5 μL full-loop injection, <90s for 5 μL partial-loop injection |
| Sample thermostatting | 4–45 °C or 22 °C below ambient (thermostatted sampler versions) |
| Sample temperature accuracy | ±2 °C |
| Dimensions (h × w × d) | 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) |
| Power requirements | 100–120 V, 60 Hz 200–240 V, 50 Hz |
| PC connection | all functions controllable via USB; integrated USB hub with 3 USB 1.1 ports (except WPS-3000(T)RS); |
| Weight: | max. 23 kg (51 lbs) including cooling |
| I/O interfaces | 4 digital inputs, 4 relay outputs |
| Ordering Information |
| WPS-3000TBFC - UltiMate 3000 Thermostatted Biocompatible Pulled-Loop Well Plate Autosampler with Integrated Fraction Collection |
5825.0020 |
| WPS-3000TFC, UltiMate 3000 Thermostatted Pulled-Loop Well Plate Autosampler with Integrated Fraction Collection |
5824.0020 |