Dionex AminoTrap Column
The Thermo Scientific™ Dionex™ AminoTrap™ is an in-line pretreatment column installed in place of the guard column, before a carbohydrate monosaccharide column.
- In-line sample pretreatment column removes amino acids from samples for monosaccharide analysis.
- Eliminates electrode fouling due to the presence of amino acids such as lysine.
- Different dimensions are available for different monosaccharide analytical columns.
The detection of monosaccharides and amine-containing glycoconjugates with low levels of glycosylation can be compromised from fouling of the working electrode by amino acids. Amino acids such as lysine are potential interferents without the AminoTrap, because the oxidation products inhibit detector response for later-eluting monosaccharides.
The Dionex AminoTrap is an in-line pretreatment column installed in place of the guard column, before an analytical carbohydrate monosaccharide column, such as the Thermo Scientific Dionex CarboPac™ PA20 or CarboPac PA10. Specifically designed with high selectivity for amino acids, the Dionex AminoTrap column retains the amino acids, thus allowing the monosaccharides to be eluted well before interfering amino acids. In this way, the Dionex AminoTrap column eliminates working electrode fouling for samples such as glycoprotein hydrolysates or food sources that contain lysine.
The AminoTrap is packed with a 10 µm particle size, high-capacity, anion-exchange resin that has high selectivity for hydrophobic amino acids relative to monosaccharides when using hydroxide eluents. The 4 × 50 mm Dionex AminoTrap is recommended for use with the Dionex CarboPac PA10. The 3 × 30 mm Dionex AminoTrap is recommended for use with the Dionex CarboPac PA20. The 0.4 x 35 mm Dionex AminoTrap is recommended for use with the Dionex CarboPac PA10 (0.4 mm) and Dionex CarboPac PA20 (0.4 mm).
Effect of the AminoTrap Column on Monosaccharide Composition Analysis of IgG Hydrolysate.
Profiling MAb Hydrolysate on the CarboPac PA20, with and without an AminoTrap.
|CarboPac PA1 and PA10 Columns for Mono- and Disaccharide Analysis Data Sheet|
|CarboPac PA20 Column Data Sheet|
|AminoTrap Column Manual|
|CarboPac PA20 Analytical Column Manual|
|CarboPac MA1, PA1, PA10, and PA100 Analytical Columns Manual|
|TN 40: Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent Generation|
|Amino Trap Column (4 x 50 mm)||046122|
|Amino Trap Column (3 x 30 mm)||060146|
|AminoTrap Column (2 x 50 mm)||SP5578|