MAbPac SCX-10 Monoclonal Antibody Analysis Column
MAbPac SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody Variants
The Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of MAb variant samples.
The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of monoclonal antibodies and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of monoclonal antibody variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.
• Unequalled high-resolution separations
• Exceptionally high efficiency
• Highest resolution of monoclonal antibody variants
• Unmatched column-to-column and lot-to-lot reproducibility
• Elimination of hydrophobic interactions
• Ideal for stability testing
The MAbPac resin technology is the basis for the superior performance of monoclonal antibody variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening.
The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher resolution separations.
MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.
The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific™ ProPac™ WCX-10 column for monoclonal antibody variant analysis. The MAbPac SCX-10 offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most monoclonal antibody samples than the ProPac WCX-10 column.
New MAbPac SCX Column Kits for Validation Now Available
- Three MAbPac SCX-10, 4 × 250 mm columns from one lot of resin
- Three MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resin
These MAbPac SCX-10 columns kits will facilitate the validation process of MAbPac SCX-10 columns for monoclonal antibody therapeutics development projects.
The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of monoclonal antibody variant separations.
Comparison of monoclonal antibody separation using the ProPac WCX-10, 10 μm, MAbPac SCX-10, 10 μm, and MAbPac SCX-10, 3 μm columns.
Fast characterization of C-terminal lysine truncation variants using the MAbPac SCX-10, 3 μm column.
The MAbPac SCX column provides excellent peak efficiencies and exceptional resolution of monoclonal antibody variants.
The MAbPac SCX-10 column provides exceptionally high resolution and peak efficiencies of monoclonal antibody acidic, basic, and C-terminal lysine variants.
The MAbPac SCX-10 column provides exceptionally high resolution and peak efficiencies of monoclonal antibody acidic and basic variants.
Baseline resolution of C-terminal lysine variants of a monoclonal antibody sample. A second chromatogram verifies that the three major peaks are due to variations in C-terminal content: after the treatment with carboxypeptidase B, only one major peak remains.
Comparison of monoclonal antibody separation on ProPac WCX and MAbPac SCX Columns.
|MAbPac SCX-10, 3µm, Analytical Column (4 x 50 mm)||077907|
|MAbPac SCX-10, 5µm, Analytical Column (4 x 50 mm)||078656|
|MAbPac SCX-10, 5µm, Analytical Column (4 x 250 mm)||078655|
|MAbPac SCX-10, 10 µm, Analytical Column (4 x 250mm)||074625|
|MAbPac SCX-10, 10µm, Analytical Column (4 x 150mm)||075602|
|MAbPac SCX-10HT, 10µm, Analytical Column (4 x 50mm)||075603|
|MAbPac SCX-10, 10µm, Analytical Column (2 x 250mm)||075604|
|MAbPac SCX-10, 10µm, 9 x 250 MM column||SP6866|
|MAbPac SCX-10, 10µm, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)||SP6864|
|MAPac SCX-10, 10µm, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)||SP6865|
|MAbPac SCX-10G, 10µm, Guard Column (4 x 50 mm)||074631|
|MAbPac SCX-10G, 10µm, Guard Column (2 x 50 mm)||075749|
|MAbPac SCX-10 Column for Monoclonal Antibody Variant Analysis and Characterization|
|Monoclonal Antibody Characterization|
|MAbPac SCX-10 Analytical Column Product Manual|
|AU 188: High-Resolution Cation-Exchange Alternative to Peptide Mapping for Protein ID and QA/QC|
|MAbPac SCX-10 Monoclonal Antibody Analysis Column Specifications|
10 μm: 4 × 250mm, 4 × 150mm, 4 ×50mm, 2 × 250mm
5 μm: 4 × 250mm, 4 ×50mm
3 μm: 4 × 50mm
|Bead Size||Uniform 10 μm, 5μm, 3 μm particles|
|Substrate||Highly crosslinked DVB media|
|Pellicular Layer||Proprietary Hydrophilic|
|Flow Rate Range:|
0.2 to 2 mL/min. for: 4 × 250mm, 4 × 150 mm, 4 × 50 mm
0.1 to 0.5 ml/min. for: 2 × 250 mm
3,000 psi for 10 μm: 4 × 250mm, 4 × 150 mm, 4 × 50 mm
7,000 psi for 3 μm and 5 μm
|Temperature Range:||Ambient to 60 ºC|
|pH Range||2 -12|
|Typical buffers:||Sodium or potassium salts of phosphate, MES|
|Solvents:||50% acetonitrile if needed for cleaning|
|Short Term Storage Solution (overnight):||Your low salt concentration eluent (pH 3-10)|
|Shipping Solution / Long Term Storage Solution:||20 mM MES (pH 5.6) + 60 mM NaCl–+ 0.1% sodium azide|
|Detergent Compatibility||Nonionic, anionic, or zwitterionic detergents.|