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Home > Dionex Products > Columns > Bio Columns > Peptide Columns > Acclaim PepMap Protein and Peptide Columns > Acclaim PepMap300 C18 Protein and Peptide Column
Acclaim PepMap300 C18 Protein and Peptide Column
Acclaim® PepMap300 C18 is used for the separation of larger peptides and proteins. It is available in 5 µm particle sizes, with 300 Å pore sizes, and in nano, capillary, and micro formats.
| Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
|---|---|---|---|
| Column type | Nano | Capillary | Micro |
| Column i.d. | 75 µm | 300 µm | 1.0 mm |
| Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
| Packing Material | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm |
| C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | |
| C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | |
| C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | |
| C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | |
| Column | Fused-silica | Glass Lined Tubing | Stainless steel |
| Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| MS Outlet | 1-m Length | ||
| Technical Notes | |
|---|---|
 |
TN 60: UltiMate 3000: Direct Sample Injection onto a 75-um I.D. PepMap 100 (C18) Column |
|
TN 61: UltiMate 3000 — Preconcentration on a 75-μm i.d. x 1 5 cm PepMap 1 00 (C18) Nanocolumn |
| Product Data Sheets | |
|---|---|
|
Acclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Data Sheet |
|
PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Data Sheet |
| Nano LC Columns | |
|---|---|
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 75 µm x 5 cm | 163577 |
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 75 µm x 15 cm | 163574 |
| Capillary LC Columns | |
|---|---|
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 300 µm x 5 cm | 163580 |
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 300 µm x 15 cm | 163581 |
| Micro LC Columns | |
|---|---|
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 1.0 mm x 5 cm | 163584 |
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 1.0 mm x 15 cm | 163585 |
- LC Modules
