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Acclaim PepMap100 C8 Peptide Column

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• Specifications
• Related Literature
• Ordering Information

Acclaim^® PepMap100 C8 is an excellent alternative for the Acclaim PepMap100 C18, when separating very hydrophobic peptides (e.g., non-tryptic peptides). It is available with 3 or 5 µm particles, with 100 Å pore sizes, and in capillary and micro formats.

Acclaim PepMap for TFA-Free Peptide Mapping  Specifications
Column type	Nano	Capillary	Micro
Column i.d.	75 µm	300 µm	1.0 mm
Length	50, 150, 250, and 500 mm	50, 150, and 250 mm	50, 150, and 250 mm
Recommended Flow Rate	200 nL/min	4 µL/min	40 µL/min
Packing Material	C18, 100 Å, 3 µm	C18, 100 Å, 3 µm	C18, 100 Å, 3 µm
	C18, 300 Å, 5 µm	C18, 300 Å, 5 µm	C18, 300 Å, 5 µm
	C8, 100 Å, 3 µm	C8, 100 Å, 3 µm	C8, 100 Å, 3 µm
	C8, 100 Å, 5 µm	C8, 100 Å, 5 µm	C8, 100 Å, 5 µm
	C4, 300 Å, 5 µm	C4, 300 Å, 5 µm	C4, 300 Å, 5 µm
Column	Fused-silica	Glass Lined Tubing	Stainless steel
Column Inlet	1/16-in. PEEK sleeve adapter (provided) nanoViper (optional)	1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings	1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
Column Outlet	Fused-silica 25-µm i.d./ 280-µm o.d.	Fused-silica 75-µm i.d./ 280-µm o.d	1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
MS Outlet	1-m Length

Application Notes
	AN 531: Phosphopeptide Enrichment Using a TiO2 Nano Precolumn
	AN 527: Comprehensive 2-D Nano LC/MS for Human Tissue Proteomics
	AN 524: Parallel Nano and Capillary LC for High-Throughput MS Proteomics
	AN 521: Automated 2D LC Coupled to ESI-MS/MS for the Analysis of Complex Peptide Samples

Technical Notes
	TN 60: UltiMate 3000: Direct Sample Injection onto a 75-um I.D. PepMap 100 (C18) Column
	TN 61: UltiMate 3000 — Preconcentration on a 75-μm i.d. x 1 5 cm PepMap 1 00 (C18) Nanocolumn

Product Data Sheets
	Acclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Data Sheet
	PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Data Sheet

Nano LC Columns
Acclaim PepMap100, C8, 3 µm, 100 Å, 75 µm i.d. x 5 cm	161184
Acclaim PepMap100, C8, 3 µm, 100 Å, 75 µm i.d. x 15 cm	161185
Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 5 cm	161555
Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 15 cm	161553
Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 25 cm	161186

Capillary LC Columns
Acclaim PepMap100, C8, 3 µm, 100 Å, 300 µm i.d. x 5 cm,	161181
Acclaim PepMap100, C8, 3 µm, 100 Å, 300 µm i.d. x 15 cm	161182
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 5 cm	161547
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 15 cm	161545
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 25 cm	161183

Micro LC Columns
Acclaim PepMap100, C8, 3 µm, 100 Å1.0 mm i.d. x 5 cm	160240
Acclaim PepMap100, C8, 3 µm, 100 Å, 1.0 mm i.d. x 15 cm	161179
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 5 cm	161539
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 15 cm	161537
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm	161180

• LC Systems
  • UltiMate 3000 Proteomics MDLC System
  • UltiMate 3000 RSLCnano System

• LC Modules
  • LC Pumps
  • Probot MALDI Spotter