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Acclaim PepMap100 C18 Protein and Peptide Column

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• Specifications
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• Ordering Information

The Acclaim^® PepMap100 C18 column sets the benchmark in peptide separation. The PepMap100 C18 column is available in 3 or 5 µm particle sizes, with 100 Å pore sizes, and in nano, capillary, and micro formats. nanoViper fingertight fittings allow for easy column installation.

 

Acclaim PepMap for TFA-Free Peptide Mapping  Specifications
Column type	Nano	Capillary	Micro
Column i.d.	75 µm	300 µm	1.0 mm
Length	50, 150, 250, and 500 mm	50, 150, and 250 mm	50, 150, and 250 mm
Recommended Flow Rate	200 nL/min	4 µL/min	40 µL/min
Packing Material	C18, 100 Å, 3 µm	C18, 100 Å, 3 µm	C18, 100 Å, 3 µm
	C18, 300 Å, 5 µm	C18, 300 Å, 5 µm	C18, 300 Å, 5 µm
	C8, 100 Å, 3 µm	C8, 100 Å, 3 µm	C8, 100 Å, 3 µm
	C8, 100 Å, 5 µm	C8, 100 Å, 5 µm	C8, 100 Å, 5 µm
	C4, 300 Å, 5 µm	C4, 300 Å, 5 µm	C4, 300 Å, 5 µm
Column	Fused-silica	Glass Lined Tubing	Stainless steel
Column Inlet	1/16-in. PEEK sleeve adapter (provided) nanoViper (optional)	1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings	1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
Column Outlet	Fused-silica 25-µm i.d./ 280-µm o.d.	Fused-silica 75-µm i.d./ 280-µm o.d	1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
MS Outlet	1-m Length

Application Notes
	AN 531: Phosphopeptide Enrichment Using a TiO2 Nano Precolumn
	AN 527: Comprehensive 2-D Nano LC/MS for Human Tissue Proteomics
	AN 524: Parallel Nano and Capillary LC for High-Throughput MS Proteomics
	AN 521: Automated 2D LC Coupled to ESI-MS/MS for the Analysis of Complex Peptide Samples

Technical Notes
	TN 60: UltiMate 3000: Direct Sample Injection onto a 75-um I.D. PepMap 100 (C18) Column
	TN 61: UltiMate 3000 — Preconcentration on a 75-μm i.d. x 1 5 cm PepMap 1 00 (C18) Nanocolumn

Data Sheets
	Acclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Data Sheet

Nano LC Columns
Acclaim PepMap100, C18, 3 µm, 100 Å, 75 µm i.d. x 5 cm	160316
Acclaim PepMap100, C18, 3 µm, 100 Å, 75 µm i.d. x 15 cm	160321
Acclaim PepMap100, C18, 3 µm, 100Å, 75 µm i.d. x 25 cm	164261
Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 5 cm	160318
Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 15 cm	160323
Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 25 cm	160326
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper	164567
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 15 cm, nanoViper	164568
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 25 cm, nanoViper	164569
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 50 cm, nanoViper	164570

Capillary LC Columns
Acclaim PepMap100, C18, 3 µm, 100 Å, 300 µm i.d. x 5 cm	160290
Acclaim PepMap100, C18, 3 µm, 100 Å, 300 µm i.d. x 15 cm	160295
Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 5 cm	160292
Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 15 cm	160297
Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 25 cm	160300
Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm i.d. × 15 cm, nanoViper	164571

Micro LC Columns
Acclaim PepMap100, C18, 3 µm, 100 Å, 1.0 mm i.d. x 5 cm	160277
Acclaim PepMap100, C18, 3 µm, 100 Å, 1.0 mm i.d. x 15 cm	160282
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 5 cm	160279
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 15 cm	160284
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm	160287
Acclaim PepMap100, C18, 3 μm, 100 Å, 1 mm i.d. × 15 cm, nanoViper	164572

• LC Systems
  • UltiMate 3000 Proteomics MDLC System
  • UltiMate 3000 RSLCnano System

• LC Modules
  • LC Pumps
  • Probot MALDI Spotter