HPLC Analysis of Nucleic Acids

The DNAPac^® series of polymer-based, anion-exchange columns set the standard for oligonucleotide purity analysis, fast screening, and purification. Two column types are available: DNAPac PA100 and PA200. Both are strong anion-exchange columns developed for high-resolution analysis and purification of synthetic and modified oligonucleotides. The DNAPac PA200 offers improved efficiency, and enhanced stability under alkaline conditions.

• High resolution separation of oligonucleotides and nucleic acids
• Capable of n, n-1 resolution for oligonucleotides
• Compatible with solvent, high pH, and high temperatures
• Provides easy scale-up

These columns support screening of synthetic oligonucleotides for production yield and failure sequences on a routine basis; unit-base resolution of synthetic oligonucleotides has been demonstrated for up to 60 bases and beyond. These columns provide unsurpassed high-resolution of full length from n–1, n+1, and other failure sequences not possible with other columns.