For Sensitive Analysis of Mono- and Disaccharides
The CarboPac® PA10 is an ideal choice for separations of mono- and disaccharides found in mammalian glycoproteins. Fucose is well resolved from the system void and from protein hydrolysate interferents. The CarboPac PA10 allows oxygen to elute after mannose, eliminating interference with monosaccharides. It is ideal for the analysis of mono- and disaccharides in foods, drugs, and plants, and separates sialic acids with the addition of sodium acetate to the eluent.
- For the quantification of acidic, neutral, and aminomonosaccharides
- Simple, isocratic separations
- Superior selectivity for common monosaccharides compared to the CarboPac PA1
- No derivatization required
- Gradient compatible
The CarboPac PA10 is an anion-exchange column designed specifically for high-resolution separations of mono- and disaccharides. Separation is accomplished with a simple isocratic eluent, and detection is accomplished with pulsed amperometric detection (PAD). No derivatization is required.
| CarboPac PA10 Column for Monosaccharide and Disaccharide Analysis Specifications |
|---|
| Dimensions | CarboPac PA10 Analytical Column:
4 × 250 mm and 2 × 250 mm
CarboPac PA10 Guard Column:
4 × 50 mm and 2 × 50 mm |
| Maximum operating pressure | 3500 psi (24.5 MPa) |
| Temperature range | 4–55 °C
Recommended operating temperature: ambient |
| Typical operating conditions | 1900 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | pH 0–14; up to 90% of common HPLC solvents.
Avoid anionic detergents and 100% water.
Acetate or hydroxide eluents only. |
| Resin composition | 10-µm-diameter substrate (ethylvinylbenzene 55% cross-linked with divinylbenzene) agglomerated with 460-nm MicroBead™ difunctional quaternary ammonium ion (5% cross-linked). |
| Anion-exchange capacity | Approximately 100 µeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic. |
High-sensitivity analysis using the CarboPac PA10.
Sialic acid analysis of bovine transferrin on the CarboPac PA10.
The CarboPac PA10 resolves mixtures of glycols, sugar alcohols, and carbohydrates in a pharmaceutical formulation.
| Analytical Columns |
|---|
| CarboPac PA10 Analytical Column (2 x 250 mm) |
057180 |
| CarboPac PA10 Analytical Column (4 x 250 mm) |
046110 |
| CarboPac PA10 Semi-Preparative (9 X 250 mm) |
SP4216 |
| Guard Columns |
|---|
| CarboPac PA10 Guard Column (2 x 50 mm) |
057181 |
| AminoTrap Column (2 x 50 mm) |
SP5578 |
| CarboPac PA10 Guard Column (4 x 50 mm) |
046115 |
| Amino Trap Column (4 x 50 mm) |
046122 |
| Borate Trap Column |
047078 |
| Amino Trap Column (3 x 30 mm) |
060146 |
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