GlycanPac AXH-1 Glycan Analysis Column
GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of Glycans
The Thermo Scientific™ GlycanPac™ AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-MS methods.
The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).
- Unique glycan selectivity based on charge, size, and polarity
- Excellent resolution for both native and labeled glycans
- Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
- Compatible with fluorescence and MS detection methods
- High chromatographic efficiency and excellent column stability
The GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and HILIC retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.
GlycanPac AXH-1 columns are based on high purity and spherical silica substrates in both 1.9 and 3 µm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.
The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. The glycan elution profile consists of a series of peaks grouped into several "clusters" in which the neutral glycans elute first, followed by mono-sialylated, di-sialylated, tri-sialylaled species.Analytes in each cluster, retained by anion exchange interactions, represents the glycans of the same charge. Within each cluster the glycans having the same charge are further separated according to their sizes and polarity by HILIC interaction.
Similar separation of N-glycans using a 3µm packing material with Fluorescence detection.
Separation of N-glycans using the GlycanPac AXH-1, 1.9µm packing with LC/MS detection.
Accurate and easy quantitative analysis of relative amounts of glycans of each charge state is possible by using a modified gradient.
Separation of human IgG using the GlycanPac AXH-1, 1.9µm column.
|GlycanPac AXH-1, 3 µm, Analytical (4.6x150mm)||082468|
|GlycanPac AXH-1, 3 µm, Analytical (3.0x150mm)||082469|
|GlycanPac AXH-1, 3 µm, Analytical (2.1x150mm)||082470|
|GlycanPac AXH-1, 1.9 µm, Analytical (2.1x250mm)||082521|
|GlycanPac AXH-1, 1.9 µm, Analytical (2.1x150mm)||082472|
|GlycanPac AXH-1, 1.9 µm, Analytical (2.1x100mm)||082473|
|GlycanPac AXH-1, 1.9 µm, Analytical (1.0 × 150 mm, with nanoViper)||164863|
|GlycanPac AXH-1, 3 µm, Guard (4.6x10 mm) 2ea (Use Holder V-2)||082474|
|GlycanPac AXH-1, 3 µm, Guard (3.0x10 mm) 2ea (Use Holder V-2)||082475|
|GlycanPac AXH-1, 3 µm, Guard (2.1x10 mm) 2ea (Use Holder V-2)||082476|
|Acclaim SST Guard Cartridge Holder V-2||069580|
|Acclaim Guard Kit (Holder and coupler) V-2||069707|
|Guard to Analytical Column Coupler V-2||074188|
|GlycanPac AXH-1 Column|
|Thermo Scientific GlycanPac AXH-1 Columns Product Manual|
|GLYCANPAC AXH-1 COLUMN Quick Start|
|Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies|
|GlycanPac AXH-1 Column Specifications|
1.9 μm: 2.1 × 250mm, 2.1 × 150mm, 2.1 ×100mm
3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm
|Bead Size||Spherical 1.9 μm, 3 μm particles|
|Substrate||High-purity, Porous, Spherical Silica|
|Functional Group||WAX and HILIC Mixed-Mode|
|Recommended Flow Rate Range:|
1.9 μm: 0.1 to 0.4 mL/min. for: all 2.1 mm columns
3 μm: 0.6 to 1.2 ml/min for 4.6 × 150 mm
0.3 to 0.6 ml/min for 3.0 × 150 mm
0.1 to 0.5 ml/min. for 2.1 × 150 mm
1.9 μm: 7,000 psi for 2.1 × 100mm,
10,000 psi for 2.1 ×150mm
15,000 psi for 2.1 × 250mm
3 μm: 6,000 psi for all 3 μm columns
|Temperature Range:||Ambient to 60 ºC|
|pH Range||2.0 - 8.0|