As researchers continue to look for greater temporal and spatial resolution with lower-volume, microdialysis perfusate samples, the ability to measure multiple analytes and receptor types simultaneously provides a distinct advantage. Dionex HPLC systems for neuroscience make it easy to quantitate multiple neurotransmitters, in a single sample, so you can glean the most information about multiple, interesting pathways from a single analysis.

Using Dionex LC systems and the Coulochem^® III along with our proven applications for analysis in neuroscience research will help you:

• Gain high-sensitivity results
• Reduce method development time
• Increase throughput
• Conserve precious samples and reagents

Monoamines & Metabolites

Whether you are using tissue or microdialysis perfusates to learn the effects of disease, drugs, or studying central neurotransmission, you need precise, reproducible results, and you need them fast. The UltiMate^®–Coulochem III system for neuroscience are optimized for high sensitivity analysis of monoamines and metabolites from tissue, microdialysis and cell culture samples.

Acetylcholine

This monoamine is the most common neurotransmitter. Our in-line, solid-phase reactor and sensitive electrochemical detection technology allows for detection limits as low as 50 fmol for acetylcholine in microdialysis samples collected in the presence of low levels of inhibitor.

Neuroactive Amino Acids

Amino acids are the most abundant group of substances that provide excitatory and inhibitory effect in the CNS. Study of low levels of these amino acids from basal striatal microdialysis perfusates can be accomplished in under 13 minutes. Achieving this fast turn around time is simplified using our fully automated UltiMate system with a Coulochem III electrochemical detector and the 6011 ultra analytical cell with unique dual coulometric sensing electrodes.

Free D-Serine and D-Aspartic Acid in Tissue

These two amino acids enantiomers can now be easily quantitated from tissue homogenates, even in the presence of large amounts of their corresponding L-enantiomers.  This method involves simple sample extraction, followed by LC-fluorescence detection after precolumn derivatization.