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Vitamin D2 and D3
Vitamins D2 (cholecalciferol) and D3 (ergocalciferol) are two forms of an essential vitamin. They are used in fortified milk, dietary supplements, and various other foods. The Acclaim WAX-1 column, operated in HILIC mode, separates these closely related substances by a different mechanism than reversed-phase columns, and may be useful as a complementary column.
Determination of vitamins D2 and D3 using an Acclaim mixed-mode column and UV absorbance detection at 285 nm.
