Dionex - Protein Therapeutics Applications Notebook

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Protein Therapeutics Applications Notebook

Overview
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Protein and monoclonal antibody therapeutics are the rising stars in the pharmaceuticals industry. This Application Notebook describes Dionex’s intelligent HPLC solutions that help to increase throughput and automate multistep workflows. It also contains a collection of application notes that describe Dionex’s best-in-class column chemistries that address every facet of protein and antibody therapeutics R&D and QA/QC challenges to ensure safe biopharmaceuticals.

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Brochures
	Monoclonal Antibody Characterization

Charge Variants Analysis by IEX Methods
	AN 127: Analysis of Monoclonal Antibody Heterogeneity by Cation-Exchange Chromatography: Separation of C-Terminal Lysine Variants
	AN 128: Monitoring Stability of Monoclonal Antibodies by Cation-Exchange Chromatography
	AN 125: Monitoring Protein Deamidation by Cation-Exchange Chromatography
	AN 126: Determination of Hemoglobin Variants by Cation-Exchange Chromatography
	AN 214: Separation of Protein Phosphoisoforms Using Strong Anion-Exchange Chromatography
	AN 129: Separation of Tryptophan and Methionine Oxidized Peptides from Their Unoxidized Forms
	AB 101: High-Resolution Cation-Exchange Chromatography for Analysis of Protein Purity and Microheterogeneity
	CAN: 110 Rapid Analysis of Recombinant Protein Production During Fermentation Using Dionex ProPac SCX Columns

Variants Analysis by HIC Methods
	AN 211: Hydrophobic Interaction Chromatography for Separation of Tryptophan and Methionine Oxidized Peptides from Their Native Forms
	High Resolution Silica Hydrophobic Interaction Chromatography (HIC) Column for Protein/Peptide Separations with Improved Hydrolytic Stability

Antibody and His-Tag Protein Separations by IMAC Methods
	CAN 107: Rapid His-Tag Purification of Recombinant Proteins Using Dionex ProPac IMAC Columns
	AN 177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using Immobilized Metal Affinity Chromatography (IMAC)
	Article: Separation of His-tag Protein Aggregation Variants on the New Analytical ProPac IMAC-10 Column
	High Resolution Protein and Peptide Separations on a Nanoengineered Analytical IMAC Column

Purity Analysis by RP Methods
	AN 230: Purity Analysis of Synthetic Thymosin Alpha 1 by Reversed-Phase HPLC with an Acclaim 300 C18 Column

Peptide Mapping
	AN 99: Peptide Mapping of Ovalbumin Using Reversed-Phase High-Performance Liquid Chromatography and Prediction of Phosphopeptide Elution
	UHPLC-MS/MS to Monitor Modifications in Biopharmaceuticals

High Throughput and Automated Methods for Antibody Screening
	Development of an Automated Method for Monoclonal Antibody Purification and Analysis
	Should Your Autosampler Just Sit and Wait? Intelligent Instrument Control Allows Sharing HPLC Modules Across Systems

Benefits of Biocompatible HPLC for Biomolecules Separation
	Analysis of Monoclonal Antibody Charge Heterogeneity Using Ion-Exchange Chromatography on a Fully Biocompatible HPLC System
	Optimal Method Development for Separation of Monoclonal Antibodies Using ProPac Weak Cation-Exchange (ProPac WCX) Columns: Effect of Iron on Column Performance

Biopharmaceutical Glycan Analysis Using High-Performance Anion-Exchange Chromatography (HPAE-PAD)
	AN 215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column
	AN 105: Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography
	AU 181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD
	AU 180: Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD
	TN 53: Determination of Glycoprotein Monosaccharide Composition by HPAE-PAD Using On-Line Electrolytically Generated Eluents
	TN 42: Glycoprotein Oligosaccharide Analysis Using High-Performance Anion-Exchange Chromatography
	TN 36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD
	TN 20: Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

Posters
	Intelligent Design of the Ion-Exchange Analytical Column for Monoclonal Antibody Chracterization
	Strategies for Coupling of Nonvolatile Salt-Based HPLC to MS Detection for the Separation and Characterization of Related Substances in Biopharmaceuticals
	Impact of Polymeric Ion-Exchange Columns’ Designs on the Separation of Proteins
	Exploration of pH-Gradient Ion-Exchange Chromatography for High-Resolution Protein Separations in Biotechnology and Proteomics
	AU 183: Separation of Peptides from Enzymatic Digestion on Different Acclaim Columns: A Comparative Study

Markets
- Biopharmaceuticals
- Pharmaceuticals

Liquid Chromatography
- UltiMate 3000 RSLCnano System
- UltiMate 3000 Proteomics MDLC System

Columns
- Protein Columns
- Peptide Columns

Genomic Sciences
Protein Sciences
Carbohydrate Sciences
Metabolomics
Medical Sciences
Neuroscience