Live Webcast: Wednesday, February 29, 2012 at 11:00 a.m. EST; 8:00 a.m. PST; 16:00 GMT
Register free at http://chromatographyonline.com/largemolecule

Event Overview:

Analyzing proteins and other large molecules presents a significant challenge to analytical scientists. There are two common approaches: top down and bottom up. The latter approach will be discussed in this presentation.

A bottom up approach is based upon breaking down the original sample, which in itself could contain many hundreds of proteins in their constituent peptides. There are several ways in which this can be achieved, and advice will be given as to which approach should be taken. Common pitfalls associated with the use of these approaches will also be discussed.

Discussion topics include one- and two-dimensional approaches while also considering the detection possibilities available to the analytical scientist for complex peptide mixture analysis, in particular the use of fast-scanning mass spectrometers. The discussion will then progress to the use of more novel phases that have been shown to have substantial advantages in the area of peptide analysis, specifically the improved orthogonality of the second dimension to the traditional ion exchange materials.

Key Learning Objectives:

• Better understanding of how to gain orthogonality with a complex sample
• How to optimize a two-dimensional separation
• How to use pepsinogen C (PGC) in the field of proteomics

Who Should Attend:

• Proteomics/Metabolomics Specialists

Presenter:

Tony Edge
Technical Manager, Chromatography
Consumables Division
Thermo Fisher Scientific, Inc.

Moderator:

Alasdair Matheson
Editor
LCGC Europe

For questions contact Jamie Carpenter at jcarpenter@advanstar.com.