Acclaim^® Mixed-Mode WAX-1 columns are packed with a new silica based stationary phase that incorporates both hydrophobic and weak anion-exchange properties.  

Separation of different types of molecules including bases, neutrals, and acids, in complicated mixtures within a single chromatographic run on the same column is challenging. The novel column chemistry of the Acclaim Mixed-Mode WAX-1 results in a multimode separation mechanism including hydrophobic, anion-exchange, and ion exclusion interactions. Consequently, retention of basic, neutral, and acidic molecules can be either independently or concurrently adjusted by changing ionic strength, pH, and organic solvent content in the mobile phase.

The Acclaim Mixed-Mode WAX-1 column features:

• Adjustable selectivity
• Selectivity orthogonal to reversed phase (RP) columns
• High capacity and unique selectivity for anionic molecules
• Simultaneous separation of acidic, basic, and neutral molecules
• Multimode retention mechanisms: reversed-phase, anion-exchange, and HILIC modes

The Acclaim Mixed-Mode WAX-1 column combines both hydrophobic and anion-exchange characteristics, which facilitates easy-to-adjust selectivity by changing mobile phase ionic strength, pH, or organic content, either independently or concurrently. Because of its unique column chemistry, the Acclaim Mixed-Mode WAX-1 column also provides “complimentary” selectivity to C18 reversed-phase columns.