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Nucleic Acid Columns

Anion-exchange chromatography is an ideal technique for the separation of nucleic acids. Nucleic acids are charged as a result of the phosphate group in the phophodiester link between the nucleotides. The regular spacing of the phosphate group along the oligonucleotide backbone makes separations based on net negative charge approximate a separation based on chain length; the longer the oligonucleotide, the longer it is retained. Ion-exchange chromatography is a higher-capacity technique than reversed-phase chromatography and is useful for purification and semipreparative applications with good recovery of activity. The DNAPac® columns provide the additional benefit of unsurpassed resolution.

DNAPac PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides

The DNAPac® PA100 is a strong anion-exchange column developed for high-resolution analysis and purification of synthetic oligonucleotides and modified oligonucleotides such as phosphorothioates. Several unique features are incorporated into the packing material to provide unit-base resolution of synthetic oligonucleotides to 60 bases and beyond. The DNAPac PA100 can be operated under denaturing conditions, such as high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5). Using the DNAPac PA100, analytical separations on the 4-mm-diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples.

DNAPac PA200 for Ultra-High-Resolution Separations of Oligonucleotides

The DNAPac® PA200 is a new, high-efficiency, strong anion-exchange column with enhanced stability under alkaline conditions compared with the DNAPac PA100. This column offers outstanding selectivity, resolution, and peak capacity when compared with existing anion-exchange columns, and allows precise control of selectivity with the use of eluent modifiers, alternate salts, and changes in pH.

   
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