| | | | Acclaim PepMap for TFA-Free Peptide Mapping | | 
Using Nano, Capillary and Micro LC Columns Acclaim® PepMapTM columns are the best choice for TFA-Free LC/MS. - C18, C8, and C4 chemistries
- Available in 100 Å and 300 Å pore size
- Superior resolution and recovery
- Excellent efficiency
- Enhanced sensitivity in LC/MS
Acclaim PepMap is a silica-based C18 stationary phase with virtually zero silanophilic activity, resulting in superior separation of peptides with minimal band broadening. Consequently, the need for TFA in peptide mapping is no longer required, allowing the use of solvent additives, such as formic acid, with substantially lower signal suppression and higher sensitivity in mass spectrometry (MS). Unsurpassed Performance Compared to other stationary phases used for peptide mapping, unsurpassed separation performance can be achieved with the Acclaim PepMap phase. Microcolumns packed with Acclaim PepMap are available from 75-µm i.d. up to 1.0-mm i.d. in different lengths and configurations to permit easy connection to MS. Acclaim PepMap Nano Columns, 75-µm i.d. (200 nL/min) - Cutting-edge miniaturized HPLC
- Ideally suited for nano LC/nanospray/MS
- Allows for analysis of minute samples
- Built for ultimate performance
- Easy to use
Nano LC, often combined with on-line mass spectrometry (MS), is the cutting-edge of microseparation techniques. Using LC Nanocolumns easily improves the mass sensitivity by a factor of up to 5000, when compared to 4.6-mm i.d. columns with conventional flow rates. Applications have proven that hyphenated techniques such as nano LC-ESI/MS gives higher detection levels as direct injection to nanospray MS. Higher detection is due to lower chemical noise and sample concentration, which lead to a better signal-to-noise ratio. Acclaim PepMap Capillary Columns, 300-µm i.d. (4 µL/min) Using Acclaim PepMap Capillary Columns, sensitivity increases dramatically, whereas solvent use and disposal decrease. Advantages include: - High efficiencies
- Savings in solvent use and disposal of up to 99%
- Outstanding column-to-column reproducibility
- Rugged and proven
- Easy on-line coupling to MS
These Capillary Columns with a 300-µm i.d. have become standard in capillary LC. Efficiencies of 100,000 plates/m for standard packings and excellent peak symmetry are achieved routinely. The universal 1/16-in. finger-tight fittings guarantee ease of use, and allow direct connection of the column to the injection valve or Microguard column. The standard stationary phase is C18 reversed-phase spherical particles with a particle size of 3 µm for 5- and 15-cm columns, and 5 µm for 25-cm columns. For easy LC/MS interface, extra-long outlet capillaries (100 cm) are available. Acclaim PepMap Micro Columns, 1.0-mm i.d. (40 µL/min) - Built for optimum ruggedness and performance
- Excellent column lifetime and column-to-column reproducibility
- Easy installation with premounted 1/16-in. tubing
These 1.0-mm i.d. microbore columns are designed to provide the user with the best column performance and stability. Low dispersion inlet and outlet tubing, with 1/16-in. o.d., allows for direct connection to injector and micro flow cell.
| | Acclaim PepMap for TFA-Free Peptide Mapping Specifications | | Column type | | | Column i.d. | | | Length | | 50, 150, and 250 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| | Recommended Flow Rate | | 200 nL/min | 4 µL/min | 40 µL/min |
| | Packing Material | C18, 100 Å,
3 µm
C18, 300 Å,
5 µm
C8, 100 Å,
3 µm
C8, 100 Å,
5 µm
C4, 300 Å,
5 µm | C18, 100 Å,
3 µm
C18, 300 Å,
5 µm
C8, 100 Å,
3 µm
C8, 100 Å,
5 µm
C4, 300 Å,
5 µm | C18, 100 Å,
3 µm
C18, 300 Å,
5 µm
C8, 100 Å,
3 µm
C8, 100 Å,
5 µm
C4, 300 Å,
5 µm |
| | Column | | Fused-silica | Glass Lined Tubing | Stainless steel |
| | Column Inlet | | 1/16-in. PEEK sleeve adapter (provided) | 1/16-in. universal fitting | 1/16-in. PEEK tubing |
| | Column Outlet | | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing |
| | MS Outlet | |
| | Order Information | | Acclaim PepMap100, C18, 3 µm, 100 Å, 75 µm i.d. x 5 cm | 160316 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 5 cm | 160318 | | Acclaim PepMap100, C18, 3 µm, 100 Å, 75 µm i.d. x 15 cm | 160321 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 15 cm | 160323 | | Acclaim PepMap100, C18, 3 µm, 100 Å, 300 µm i.d. x 5 cm | 160290 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 5 cm | 160292 | | Acclaim PepMap100, C18, 3 µm, 100 Å, 300 µm i.d. x 15 cm | 160295 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 15 cm | 160297 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 25 cm | 160300 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 5 cm | 160279 | | Acclaim PepMap100, C18, 3 µm, 100 Å, 1.0 mm i.d. x 5 cm | 160277 | | Acclaim PepMap100, C18, 3 µm, 100 Å, 1.0 mm i.d. x 15 cm | 160282 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 25 cm | 160326 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 15 cm | 160284 | | Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm | 160287 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 75 µm x 5 cm | 163577 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 75 µm x 15 cm | 163574 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 300 µm x 5 cm | 163580 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 300 µm x 15 cm | 163581 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 1.0 mm x 5 cm | 163584 | | Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 1.0 mm x 15 cm | 163585 | | Acclaim PepMap100, C8, 3 µm, 100 Å, 75 µm i.d. x 5 cm | 161184 | | Acclaim PepMap100, C8, 3 µm, 100 Å, 75 µm i.d. x 15 cm | 161185 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 5 cm | 161555 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 15 cm | 161553 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 25 cm | 161186 | | Acclaim PepMap100, C8, 3 µm, 100 Å, 300 µm i.d. x 15 cm | 161182 | | Acclaim PepMap100, C8, 3 µm, 100 Å, 300 µm i.d. x 5 cm, | 161181 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 5 cm | 161547 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 15 cm | 161545 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 25 cm | 161183 | | Acclaim PepMap100, C8, 3 µm, 100 Å1.0 mm i.d. x 5 cm | 160240 | | Acclaim PepMap100, C8, 3 µm, 100 Å, 1.0 mm i.d. x 15 cm | 161179 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 5 cm | 161539 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 15 cm | 161537 | | Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm | 161180 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 75 µm x 5 cm | 163578 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 75 µm x 15 cm | 163579 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 300 µm x 5 cm | 163582 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 300 µm x 15 cm | 163583 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 1.0 mm x 5 cm | 163586 | | Acclaim PepMap300, C4, 5 µm, 300 Å Wide Pore, 1.0 mm x 15 cm | 163587 |
| | Data Sheets | | No datasheet available at this time. |
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