| | | | Acclaim 300 Reversed-Phase Silica Columns for Protein, Peptide, and Macromolecule Separations |
| The Acclaim® 300 columns are a wide-pore line of C18 reversed-phase columns manufactured by for the high-resolution separation of proteins, peptides, and other biological macromolecules. The unique bonding chemistry of Dionex Acclaim columns show you what lies between the peaks, column after column. The Acclaim 300 was specifically designed for analysis of proteins and peptides, and features 3-µm silica particles for rapid analysis of complex protein digests. Because variations in both the raw silica and the ligand bonding chemistry and density significantly impact performance, each step of the process uses established procedures to maintain tight control. We start with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Next, taking advantage of our unique bonding chemistries, we apply exhaustive bonding and end-capping techniques. These techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interaction mechanisms. Because of the stable bonding, only insignificant bleeding occurs, making these columns compatible with LC/MS applications. The packing procedures for the Acclaim 300 ensure maximum efficiencies. The Acclaim 300 can readily resolve proteins that differ by only a few amino acid residues. The Acclaim 300 phases have very high surface coverage, resulting in high-capacity columns that are stable between pH 2 and 8. The use of a 3-µm silica particle accelerates the diffusion of the mobile phase into the stationary phase, resulting in fast, high-resolution separations. Compared to 5-µm column packings, it is possible to increase the flow rate of the mobile phase and run shallower gradients on shorter columns to achieve the desired separation in a shorter time period. To meet the exacting needs of our customers, each Acclaim column is manufactured to stringent specifications to ensure the column-to-column reproducibility. Each column is shipped with a lot validation sheet showing the test results and specifications for the lot of bonded silica packed into the column and an individual column test chromatogram validating performance. The lot validation includes a chromatography-based metals test and tests for polar selectivity, hydrophobic steric selectivity, and base asymmetry. Production columns are individually tested for capacity and efficiency, and closely monitored for metals contamination. Reproducible column-to-column manufacturing, the use of ultrapure silica, and performance validation of every column prior to shipping, all ensure that the quality of your separations will be maintained month to month and year to year.
| | Acclaim 300 Reversed-Phase Silica Columns for Protein, Peptide, and Macromolecule Separations Specifications | | USP Listing |
L1
| | Starting material |
Ultrapure silica
| | Particle size | 3 µm | | Particle shape |
Spherical
| | Particle size distribution (40/90) |
1.2–1.3
| | Total carbon content |
7–8.8%
| | Surface coverage |
3.7 mmol/m2
| | End-capped |
Fully
| | Metal impurity (Na, Fe, Al) | <10.0 ppm Total Metal
<3.0 ppm Individual Metal | | Pore volume |
0.9–1.0 mL/g
| | Average pore diameter |
260–300 Å
| | Surface area |
90–120 m2/g
| | pH range |
2–8
| | Temperature |
<60 °C
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| | Order Information | | SST Guard Cartridge Holder | 059456 | | Guard to Analytical Column Coupler | 059457 | | Guard Kit (Holder and Coupler) | 059526 | | Acclaim 300, C18, 3 µm Analytical (2.1 x 50 mm) | 060263 | | Acclaim 300, C18, 3 µm Analytical (2.1 x 150 mm) | 060264 | | Acclaim 300, C18, 3 µm (3.0 x 150 mm) | 063684 | | Acclaim 300, C18, 3 µm Analytical (4.6 x 50 mm) | 060265 | | Acclaim 300, C18, 3 µm Analytical (4.6 x 150 mm) | 060266 | | Acclaim 300 Guard Cartridges (4.3 x 10 mm), 2 Each | 060393 | | Acclaim 300 Guard Cartridges (2 x 10 mm), 2 Each | 060395 |
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|  |  | ProPac HIC-10 Columns | | The ProPac HIC-10 column is a high capacity hydrophobic interaction chromatography column for the separation of proteins under non-denaturing conditions. |
| Acclaim 120 Reversed-Phase Columns |  | The Acclaim® 120 columns are a highly characterized line of LC/MS compatible C18 and C8 phases that have very high surface coverage and very low metal content. These columns provide exceptional performance for a variety of applications in the pharmaceutical, nutraceutical, food, and life science separations areas. |
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