| | | | IonPac NS1 Polymeric Reversed-Phase Column |
| The IonPac® NS1 is a reversed-phase column, resistant to acids, bases, and solvents, that is ideal for the separation of hydrophobic, ionizable compounds such as organic acids and surfactants. The NS1 is the column of choice for MPIC® applications Ideal for the separation of large molecules that carry localized charges, such as surfactants Compatible with acids, bases, and solvent from pH 0 to 14 Can also be used for traditional polymeric reversed-phase applications
The IonPac NS1 column is the column of choice for routine ion-pair chromatography or ion-suppression separations using the AMMS® ICE suppressor. Typical ion-pair reagents for anion analysis include ammonium and tetraalkylammonium hydroxides. Typical ion-pair reagents for cation analysis include hydrochloric, perchloric, and sulfonic acids. The NS1 is also used with Mobile Phase Ion Chromatography (MPIC), which is a technique especially suited to large molecules that carry localized charges (e.g., surfactants), as well as other ions that are not amenable to separation by ion-exchange chromatography. Detergent and other chemical manufacturers routinely use MPIC for the analysis of compounds such as ionic surfactants or quaternary ammonium compounds. This technique is also used by cosmetic manufacturers for the analysis of additives such as alkanolamines, and by the pharmaceutical industry for a variety of nonchromophoric, ionic species. The ASRS® or CSRS® suppressors are required for MPIC. The IonPac NS1-10µm and NS1-5µm columns are packed with a neutral, macroporous, high-surface-area, ehtylvinylbenzene polymer cross-linked with 55% divinylbenzene. This resin makes the NS1 resistant to solvents, acids, and bases, and permits the use of eluents from pH 0 to 14. The NS1-10µm is used for routine MPIC separations. The NS1-5µm column is packed with a high-efficiency, 5-µm-diameter resin for more resolving power and greater sensitivity.
| | IonPac NS1 Polymeric Reversed-Phase Column Specifications | | Dimensions | IonPac NS1-10µm Analytical Column:
4 × 250 mm IonPac NS1-10µm Guard Column:
4 × 35 mm IonPac NS1-5µm Analytical Column:
4 × 150 mm | | Maximum operating pressure |
<4000 psi (<27.6 MPa)
| | Typical operating conditions | Gradient: 1.0 mL/min Recommended Operating Temperature: Ambient Recommended Flow Rate: 1.0 mL/min Typical Ion-Pairing Reagents: For anions: ammonium and tetramethyl-, tetraethyl-, tetrapropyl-, and tetrabutyl-ammonium hydroxides. For cations: hydrochloric, perchloric, and perfluorocarboxylic acids; pentane-, hexande-, heptane-, and octanesulfonic acids. | | Mobile phase compatibility |
pH 0–14; 1–100% HPLC solvents
| | Resin composition | Bead Diameter: 10 µm (4 × 250 mm), 5 µm (4 × 150 mm), macroporous ethylvinylbenzene/divinylbenzene resin Cross-Linking (%DVB): 55% Hydrophobicity: High | | Capacity |
60 µeq (2 × 250 mm analytical column)
| | Column construction |
PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.
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| | Order Information | | IonPac NS1-10 µm Analytical Column (4 x 250 mm) | 035321 | | IonPac NG1-10 µm Guard Column (4 x 35 mm) | 039567 | | IonPac NS1-5 µm Analytical Column (4 x 150 mm) | 039568 | | IonPac NS1 10 µm Analytical Column (2 x 250 mm) | SP4354 | | IonPac NG1-10 µm Guard Column (2 x 50 mm) | SP4356 |
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|  |  | SRS 300 |  | The Self-Regenerating Suppressor, (SRS® 300), is used for electrolytically regenerated suppression of ion chromatography applications requiring high capacity, fast start-up, and sensitivity. |
| AMMS-ICE 300 | | The AMMS®-ICE 300 is used for chemically regenerated suppression of ion chromatography eluents used in the analysis of organic acids and alcohols. |
| Ion-Pair Reagents |  | Highly purified ion-pairing reagents are used in mobile phase ion chromatography (MPIC®), combining reversed-phase ion-pair chromatography with chemical suppression. |
| Eluent Concentrates | | The IonPac® eluent concentrates are available for anion and cation determinations. The eluent concentrates are recommended for fast, reliable eluent preparation. |
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