AminoPac PA10 Column
AminoPac PA1 Column Dionex offers several columns for the anion-exchange separation of amino acids. The new AAA-Direct TM Amino Acid System, which incorporates the AminoPac® PA10, revolutionizes the determination of amino acids because amino acids are detected directly, with high sensitivity, by integrated pulsed amperometric detection (IPAD). This technique is recommended for the separation of free amino acids, for example, in fermentation broths, protein characterization studies, or food analysis. The AminoPac PA1 offers the customer a means to separate amino acids by anion-exchange separation and detect them using derivatization techniques such as OPA. For the analysis of physiological amino thiols, Dionex recommends cation-exchange chromatography using the OmniPac® PCX columns.
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| AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct | The AminoPac® PA10 is a strong anion-exchange column used, as part of the AAA-DirectTM system, for high-resolution separations of free amino acids, without derivatization. Amino sugars and carbohydrates are detected simultaneously with amino acids if they are present in the sample. In addition, separation and detection of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids can be accomplished by this means. AAA-Direct is directly compatible with all four of the commonly used protein hydrolysis procedures, and the results compare favorably with those obtained by the classical Moore and Stein Ninhydrin procedure.
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