Columns and Accessories > Bio Columns > Amino Acid Columns
 
Printer Friendly

Amino Acid Columns

AminoPac PA10 Column
AminoPac PA1 Column

Dionex offers several columns for the anion-exchange separation of amino acids. The new AAA-Direct TM Amino Acid System, which incorporates the AminoPac® PA10, revolutionizes the determination of amino acids because amino acids are detected directly, with high sensitivity, by integrated pulsed amperometric detection (IPAD). This technique is recommended for the separation of free amino acids, for example, in fermentation broths, protein characterization studies, or food analysis. The AminoPac PA1 offers the customer a means to separate amino acids by anion-exchange separation and detect them using derivatization techniques such as OPA. For the analysis of physiological amino thiols, Dionex recommends cation-exchange chromatography using the OmniPac® PCX columns.

AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct

The AminoPac® PA10 is a strong anion-exchange column used, as part of the AAA-DirectTM  system, for high-resolution separations of free amino acids, without derivatization. Amino sugars and carbohydrates are detected simultaneously with amino acids if they are present in the sample. In addition, separation and detection of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids can be accomplished by this means. AAA-Direct is directly compatible with all four of the commonly used protein hydrolysis procedures, and the results compare favorably with those obtained by the classical Moore and Stein Ninhydrin procedure.

AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization

The AminoPac® PA1 is a pellicular anion-exchange column that offers unique separation characteristics for the separation of amino acids in a variety of specialized applications. The column is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

     
  Columns and Accessories > Bio Columns > Amino Acid Columns  
 
Order Literature   Privacy   Feedback   Terms of Use   Trademarks   Site Map
© 2008 Dionex Corporation. All rights reserved. www.dionex.com