Proteomics MDLC

Looking Deeper into the Proteome

Using the unique x2 dual-gradient technology in combination with the UltiFlow™ technology for nano and capillary flow generation, the UltiMate ^® 3000 Proteomics MDLC offers a wide flow rate range and high flow flexibility. This allows the system to perform an extensive range of applications, as shown in the figure below:

UltiMate 3000 Proteomics MDLC Configurations, Applications and Column Technology.

Taking full advantage of the UltiMate 3000 Proteomics MDLC system, fully automated multidimensional LC (MDLC) is performed easily. This orthogonal separation mechanism, often using ion-exchange combined with reversed-phase chromatography, can be applied using on-line or off-line separation strategies, and provides excellent opportunities for identification of low-abundance proteins with outstanding reproducibility.

• On-line MDLC strategy: well-matched for further separation of digested 1-D gel bands or 2-D gel spots. In addition, this approach provides excellent results for the analysis of highly complex proteomic samples, such as purified biological fluids or PTM complexes.
• Fully Automated Off-line MDLC strategy: specifically designed for highly complex proteomic samples such as cell lysates and tissues. Using the advanced technology of the WPS-3000 well plate autosampler, protein pre-fractionation can be performed, and then followed by re-injection of each fraction without any manual handling of the sample. This automated approach to sample preparation allows for the identification of low-abundance proteins or biomarkers.
• 2-D LC Separations for the Analysis of Intact Proteins : for the separation of complex proteomics samples, a combination of high-resolution columns is available, allowing intact protein separations.
  

The UltiMate 3000 Proteomics MDLC system provides:

• Unique x2 dual-gradient systems that enable ion-exchange AND reverse-phase separation on one system
• The advanced UltiMate 3000 well plate sampler for both injection and fractionation, providing fully automated off-line 2-D LC and protein pre-fractionation
• Nanoflow capabilities for the final analytical stage
• Reduced solvent consumption without compromising application flexibility
• On-line fraction collection on MALDI targets with the Probot™ Microfraction Collector/Spotter
• Complete range of column types for your separation needs
• Fractionation control and visualization of 2-D retention map using Chromeleon ^® Chromatography Management Software
• DCMSLink™ for single-point control of UltiMate 3000 through Xcalibur™ (Thermo Fisher), Analyst ^®  (AB/Sciex) and HyStar™ (Bruker Daltonics) for easy ESI coupling
• Easy automation of separation processes, leading to higher sample throughput, more reliable results, and improved laboratory productivity

In order to provide the highest confidence in protein identification, especially when analyzing PTM peptides and proteins, the UltiMate 3000 Proteomics MDLC is also available in a biocompatible set-up.