| | | | Rapid Separation Autosampler |
| The Rapid Separation Autosampler is an essential component of the UltiMate® 3000 Rapid Separation LC System and can be operated at ultrahigh backpressures. Unlike other UHPLC autosamplers, the Rapid Separation Autosampler can inject sample volumes up to 100 µL at pressures up to 800 bar (11,600 psi). An optimized rotor seal design (patent pending) ensures very long life times of the rotor seal and stator even when working at continuously high pressures. The wide injection volume range of the RS sampler makes the module suitable for both UHPLC and conventional HPLC applications. For highest separation quality, the sampler offers minimal gradient delay and low extra column volumes which mean high efficiency separations and short chromatographic run times. The sampler uses an in-line, split-loop injection principle where the needle and sample loop are integral parts of the high-pressure fluidic path. This ensures 100% transfer of the sample volume and virtually eliminates carryover between sample injections. The UltiMate 3000 Rapid Separation Autosampler is strongly recommended for ultrahigh pressure, high-throughput and high-resolution applications. Supports an injection volume range from 0.001 to 100 µL (settable range) Well plate compatible sample trays paired with low injection cycle times (< 15 s for 5 µL) support high throughput LC and LC-MS applications The UltiMate Rapid Separation Autosampler have only 3.1 µL of extracolumn volume to minimize dispersion during injection. Combination of a high-precision drive mechanism and a low gradient delay volume (150 µL standard, 50 µL with micro loop) routinely delivers injector linearity values of r2 > 0.9999 at 5-90 µL injection volume Volumetric accuracy better than +0.5% for 20 µL injections guarantees consistent results between instruments and easy method portability Peak area precision is typically < 0.15% RSD for 2 µL injections
Performance Examples
| | | Rapid Separation Autosampler Specifications | | Sample capacity | 120 × 1.8 mL/2.0 mL vials, 120 × 1.1 mL conical vials, 216 × 1.2 mL vials, 216 × 0.3 mL vials, 30 × 10 mL vials and/or
66 × 4 mL vials, 3 × 96 and/or 384 normal or deep well plates + 15 × 10 mL vials (depending on sample tray configuration) | | Injection methods | In-line split-loop injections, bypass mode, user-defined programs | | Injection volume range (recommended) | 0.01–100 μL
(1–100 μL) | | Injection volume accuracy | ±0.5% at 20 μL | | Minimum sample required | 1 μL out of 5 μL (250 μL conical vial) | | Injection volume precision | < 0.25% RSD at 5 μL (typically < 0.15% RSD), caffeine in water | | Linearity | Corr. coeff. > 0.9999, RSD < 0.5% at 5–90 μL, caffeine in water | | Needle wash | Active external | | Carry over | < 0.005% for caffeine with external wash at 200 bar | | Injection cycle time | < 15 seconds for 5 µL | | Sample thermostatting | 4–45 °C or 22 °C below ambient (thermostatted sampler versions) | | Sample temperature accuracy | ±2 °C | | Power requirements | 100-120 V, 60 Hz
200-240 V, 50 Hz | | Dimensions (h x w x d) | 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.); | | PC connection | USB | | I/0 interfaces | 4 digital inputs and 4 programmable outputs |
| | Order Information | | WPS-3000RS Rapid Separation Wellplate Sampler
| 5840.0010 | | WPS-3000TRS Rapid Separation Wellplate Sampler, Thermostatted
| 5840.0020 |
| | Data Sheets | | No datasheet available at this time. |
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|  |  | Rapid Separation LC | | The UltiMate Rapid Separation LC (RSLC) system offers a unique combination of high flow rate, high pressure, and ultrafast data collection. |
| LC and LC/MS Overview | Discover the UltiMate ® 3000 LCi series, and learn how LCi solutions can improve performance and productivity in your laboratory. |
| Modules | | UltiMate ® 3000 LC modules achieve new levels of flexibility, performance, reliability, and ease of use. |
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